The assay mechanism utilizes the enzyme aldehyde dehydrogenase to quantify acetaldehyde in the sample. This enzyme also oxidizes other aldehydes present in the sample. Therefore, the assay does not distinguish between acetaldehyde and other aldehydes. Consideration should be given if high levels of other aldehydes are expected in the tested sample.
MAK434
Acetaldehyde Assay Kit
Sufficient for 100 colorimetric tests
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|
O této položce
NACRES:
NA.84
UNSPSC Code:
12161503
Informace o stanovených cenách a dostupnosti nejsou v současnosti dostupné.
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohliQuality Segment
input
cell culture medium
tissue
serum
urine
food(s)
beverage(s)
plasma
application(s)
agriculture
cosmetics
environmental
food and beverages
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
storage temp.
−20°C
General description
Acetaldehyde (CH₃CHO) is a commonly found aldehyde in nature and widely used in various industries. It is a metabolic byproduct of ethanol in the liver and is toxic to the human body. However, it is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. Individuals deficient in aldehyde dehydrogenase experience accumulation of acetaldehyde upon alcohol consumption, leading to facial and body flushing known as alcohol flush reaction or “Asian flush syndrome.” Acetaldehyde buildup has also been linked to alcohol-related hangover effects. Despite its classification as a carcinogen, acetaldehyde is naturally present in many foods and beverages such as ripe fruit, coffee, and wine.
Application
The Acetaldehyde Assay Kit may be used for:
- Alcohol Metabolism Research
- Hepatology Research
- Neurological Research
The kit is suitable for acetaldehyde determination in biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).
Biochem/physiol Actions
The Acetaldehyde Assay Kit is based on the aldehyde dehydrogenase catalyzed oxidation of acetaldehyde with the reduction of NAD to NADH. The formed NADH subsequently reduces MTT, producing a colored formazan compound. The intensity of the end product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.
Features and Benefits
- Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent and only a 30 minute assay room temperature reaction, saving you time and effort.
- Convenient and Sensitive: Uses only 20 µL of sample for the determination of acetaldehyde with a detection range of 2 µM to 2mM.
- High-Throughput Compatibility: Seamlessly integrate our kit into high-throughput handling systems, ensuring efficiency and accuracy.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
1 of 1
Tato položka | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level - |
| detection method colorimetric | detection method - | detection method - | detection method fluorometric |
| input cell culture medium | input - | input - | input culture(s) |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| application(s) agriculture | application(s) - | application(s) - | application(s) cosmetics |
| relevant disease(s) gastrointestinal diseases | relevant disease(s) - | relevant disease(s) - | relevant disease(s) gastrointestinal diseases |
Still not finding the right product?
Explore all of our products under Acetaldehyde Assay Kit
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Skladovací třída
12 - Non Combustible Liquids
Vyberte jednu z posledních verzí:
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Související obsah
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| MAK434-1KT | 04065267335506 |
-
Does this assay differentiate between formaldehyde in samples and acetaldehyde?
1 answer-
Helpful?
-
-
How should MAK434 be used for cell samples?
1 answer-
The protocol for cell samples using MAK434 is as follows:
- Suspend about two million (2 × 10^6) harvested cells in 400 μL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge the homogenate at 14,000 g for 10 min and transfer the clear supernatant into a clean tube. It is advisable to run a pilot test of the sample at different dilutions and select a dilution with readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
- If a lysis buffer is to be used, it is recommended to first run an assay to test if components in the lysis buffer interfere with the assay. This can be accomplished by running two standard curves: one prepared in H2O and one prepared in the same proportion of lysis buffer that the sample will contain. If the two standard curves are the same, there is no interference and it is safe to use the lysis buffer. If there is a difference in the standard curves, the lysis buffer may still be used if the standard curve remains linear; however, the standard curve to be used for analyzing the lysate samples will need to be prepared in the lysis buffer.Helpful?
-