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WBKLS

Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Synonyma:

HRP substrate, chemiluminescent detection substrate, detection substrate

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Vybrat velikost

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Velikost baleníSkladová položkaDostupnostCena
2 x 25 mL

Odhaduje se, že bude odesláno dnesodAreál Kühne+Nagel spol. s r.o.

2 240,00 Kč
2 x 50 mL

Odhaduje se, že bude odesláno dnesodAreál Kühne+Nagel spol. s r.o.

3 760,00 Kč
2 x 250 mL

Odhaduje se, že bude odesláno dnesodAreál Kühne+Nagel spol. s r.o.

11 600,00 Kč

O této položce

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053

2 240,00 Kč

Odhaduje se, že bude odesláno dnespodrobné informace

​
Technický servis
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conjugate

peroxidase conjugate

Quality Level

200

packaging

pkg of 250 mL Luminol, pkg of 250 mL Peroxide solution

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

5000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

General description

Immobilon Western HRP Substrate is compatible with all commonly used buffers and blocking reagents and provides high sensitivity for chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes.

Application

Immobilon Western Chemiluminescent HRP Substrate is suitable for western blotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tato položka
WBULS0500WBLUCWBULS0100
Immobilon Western Chemiluminescent HRP Substrate Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Millipore

WBKLS

Immobilon Western Chemiluminescent HRP Substrate

Immobilon ECL Ultra Western HRP Substrate Western blotting, HRP

Millipore

WBULS0500

Immobilon ECL Ultra Western HRP Substrate

Immobilon Classico Western HRP substrate Western blotting, dot/slot blotting

Millipore

WBLUC

Immobilon Classico Western HRP substrate

Immobilon ECL Ultra Western HRP Substrate Western blotting, HRP

Millipore

WBULS0100

Immobilon ECL Ultra Western HRP Substrate

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

300

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

manufacturer/tradename

Immobilon®

manufacturer/tradename

-

manufacturer/tradename

Luminata

manufacturer/tradename

-

packaging

pkg of 250 mL Luminol, pkg of 250 mL Peroxide solution

packaging

-

packaging

-

packaging

-

Skladovací třída

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Protokoly

Lýza buněk a extrakce proteinů pro Western Blotting

Protokol pro přípravu vzorků pro buněčnou lýzu a účinnou extrakci proteinů z kultivovaných tkání a buněk pro následnou Western blotting.

Odstraňování a opětovné rozmnožování membrán pro Western Blotting

Na této stránce jsou uvedeny a popsány tři protokoly pro stripování a reprobování membrány Western blotu.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Zobrazit všechny protokoly

Sortimentní položky

Dot/Slot (Filtration) Blotting

The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Související obsah

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Elizabeth I Vink et al.
Virology, 422(2), 317-325 (2011-11-30)
The adenovirus E4-ORF3 protein promotes viral replication by relocalizing cellular proteins into nuclear track structures, interfering with potential anti-viral activities. E4-ORF3 targets transcriptional intermediary factor 1 alpha (TIF1α), but not homologous TIF1β. Here, we introduce TIF1γ as a novel E4-ORF3-interacting
Nicolas Bidère et al.
Nature, 458(7234), 92-96 (2009-01-02)
The transcription factor NF-kappaB is required for lymphocyte activation and proliferation as well as the survival of certain lymphoma types. Antigen receptor stimulation assembles an NF-kappaB activating platform containing the scaffold protein CARMA1 (also called CARD11), the adaptor BCL10 and
Novel irreversible epidermal growth factor receptor inhibitors by chemical modulation of the cysteine-trap portion.
Carmi, Caterina, et al.
Journal of medicinal chemistry, 53, 2038-2050 (2010)
Zobrazit všechny související dokumenty

Globální číslo obchodní položky

Skladová položkaGTIN
WBKLS005008436037125140
WBKLS010008436037125102
WBKLS050004053252616754

Questions

  1. What is hydrogen peroxide concentration in Immobilon Western Chemiluminescent HRP Substrate, WBKLS0500?

    1 answer

    1. The concentration of the Hydrogen Peroxide component is considered proprietary, but has been optimized for use with the Luminol Reagent. Equal volumes of each component are required, as indicted in the table below.

      Please see the link below to review additional information available in the product user guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/865/668/p36599.pdf

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