The recommendation to not vortex these solutions is because it would introduce dissolved oxygen into the solution which may oxidize the luminol. Over time this would shorten the shelf life of the reagents.
WBULS0100
Immobilon ECL Ultra Western HRP Substrate
Western blotting, HRP
Synonyma:
HRP substrate
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
20 mL | K dispozici k odeslání DNESodAreál Kühne+Nagel spol. s r.o. | 1 570,00 Kč |
100 mL | K dispozici k odeslání DNESodAreál Kühne+Nagel spol. s r.o. | 7 510,00 Kč |
O této položce
UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053
Technický servis
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Dovolte nám, abychom vám pomohliQuality Segment
technique(s)
western blot: suitable
detection method
chemiluminescent
shipped in
ambient
General description
Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.
Application
Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.
Sufficient for 1000 cm²
Sufficient for 1000 cm²
Preparation Note
Store at 2–8 °C
Other Notes
Solution A- 50 mL
Solution B- 50 mL
Solution B- 50 mL
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tato položka | |||
|---|---|---|---|
| description Western blotting, HRP | description Western blotting, HRP | description Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents. | description Western blotting, dot/slot blotting |
| technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| detection method chemiluminescent | detection method chemiluminescent | detection method chemiluminescent | detection method chemiluminescent |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
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Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_c
Not applicable
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Why does the protocol say not to agitate the mixed working solution? What is the rationale or reasoning behind this?
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