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UPS2

Proteomics Dynamic Range Standard Set

Protein Mass Spectrometry Calibration Standard

Synonyma:

Dynamic Range Standards, Proteomics Standard Set

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O této položce

UNSPSC Code:
12161503
EC Number:
232-650-8
NACRES:
NA.24
Form:
ready-to-use solution
Biological source:
human
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biological source

human

Quality Level

form

ready-to-use solution

quality

Protein Mass Spectrometry Calibration Standard

concentration

10.6 μg/ampule protein

technique(s)

mass spectrometry (MS): suitable

shipped in

wet ice

storage temp.

−20°C

General description

The Proteomics Dynamic Range Standard Set is produced from a mixture of 47 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.

Application

Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes. It has also been used as a standard to spike HeLa cells for label-free quantification.
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[2] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[1]

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Tato položka
UPS1EMS0004EMS0005
technique(s)

mass spectrometry (MS): suitable

technique(s)

mass spectrometry (MS): suitable

technique(s)

-

technique(s)

-

biological source

human

biological source

-

biological source

-

biological source

-

concentration

10.6 μg/ampule protein

concentration

-

concentration

-

concentration

-

form

ready-to-use solution

form

ready-to-use solution

form

ready-to-use solution

form

ready-to-use solution

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C


Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list & Stanovení ceny

  • Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg
    Bezpečností list

signalword

Danger

target_organs

Respiratory system

Skladovací třída

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3



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Osvědčení o analýze (COA)

Lot/Batch Number

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Související obsah

Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.

High-throughput proteomics advances with improved analysis methods and mass spectrometry.


Erik Ahrné et al.
Proteomics, 13(17), 2567-2578 (2013-06-25)
There is a great interest in reliable ways to obtain absolute protein abundances at a proteome-wide scale. To this end, label-free LC-MS/MS quantification methods have been proposed where all identified proteins are assigned an estimated abundance. Several variants of this
An interaction landscape of ubiquitin signaling
Zhang X, et al.
Molecular Cell, 65(5), 941-955 (2017)
Boumediene Soufi et al.
Frontiers in microbiology, 6, 103-103 (2015-03-06)
We set out to provide a resource to the microbiology community especially with respect to systems biology based endeavors. To this end, we generated a comprehensive dataset monitoring the changes in protein expression, copy number, and post translational modifications in



Globální číslo obchodní položky

Skladová položkaGTIN
UPS2-5X1SET04061837410352
UPS2-1SET04061837410345

Questions

  1. Regarding SKU# UPS2, can you please send me FASTA file of 48 proteins in UPS2 product? thanks you

    1 answer
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