T6567

Trypsin Protease

Hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, from Porcine pancreas

• Synonyma: Porcine Trypsin, Trypsin for Mass Spectropetry
• UNSPSC Code: 12352204
• NACRES: NA.56
• EC Number: 232-650-8
• MDL number: MFCD01775455
• Biological source: Porcine pancreas

Vlastnosti

• Název produktu: Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated
• biological source: Porcine pancreas
• Quality Segment: 200
• product line: BioReagent
• solubility: 1 mM HCl: soluble 1 mg/mL, clear, colorless
• shipped in: wet ice
• storage temp.: 2-8°C

Popis

General description

Trypsin is a serine protease that is usually used in biochemistry and biology as an important enzymatic reagent. This method produces a highly purified trypsin product suitable for proteomics research. Proteomics Grade ideal for use in both solution and in-gel tryptic digestions. Trypsin, a serine protease, is present in the digestive system of several vertebrates.

Application

Trypsin from porcine pancreas is used for the following applications:

• In-gel protein digestion and MALDI-TOF mass spectrometry analysis
• Electrospray Ionization Mass Spectrometry (ESI-MS) analysis
• Surface proteome profiling of L. plantarum
• Gel Filtration, Ultracentrifugation, and Rotary Shadowing Electron Microscopy
• Mass spectrometry

Biochem/physiol Actions

Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work, due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.. The enzyme also exhibits esterase and amidase activities. Trypsin acts as a cell culture tool. It is used to hydrolyze allergenic proteins to produce hypoallergenic milk in industries.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Bezpečnostní informace

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Skladovací třída: 11 - Combustible Solids
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves