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R0883

RPMI-1640 Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

RPMI-1640 Medium

Synonyma:

Roswell Park Memorial Institute 1640 medium

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Vám/Skladová položkaDostupnostCena
100 mL
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411,00 Kč
500 mL
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1 090,00 Kč
1 L
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1 510,00 Kč
6 x 500 mL
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6 160,00 Kč
6 x 1 L
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8 180,00 Kč
24 x 500 mL
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19 400,00 Kč

O této položce

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

411,00 Kč

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Název produktu

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells[1]
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
  • to cultivate circulating tumor cells (CTCs)[2]

Preparation Note

Supplement with 0.3 g/L L-glutamine.

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Tato položka
R7638R7509R8758
RPMI-1640 Medium With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R0883

RPMI-1640 Medium

RPMI-1640 Medium Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R7638

RPMI-1640 Medium

RPMI-1640 Medium Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R7509

RPMI-1640 Medium

RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R8758

RPMI-1640 Medium

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Skladovací třída

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

  1. Could you tell me the exact concentration of glucose in this RPMI-1640 medium(cat#R0883 ), please ?

    1 answer

    1. For this RPMI formulation, the glucose concentration is 2 grams per liter.

      Please see the datasheet below which includes the formulations of all classical RPMI-1640 media products:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/229/770/r0883for.pdf

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1 Review This action will navigate to reviews.
1 out of 1 (100%) reviewers recommend this product

Reviews

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  1. Boston, MA
    • Reviews 3
    • Votes 12
    5 out of 5 stars.

    Great product!

    Reliable cell culture product that gets frequent use

    Helpful?