Protein A Sepharose CL-4B exhibits high chemical and mechanical stability, and is stable over the pH range 2-11. It withstands high concentrations of denaturants, such as urea and guanidine hydrochloride. The gel displays high thermal stability but is not autoclavable. Furthermore, the gel can, if handled with care, be used many times.
P3391
Protein A-Sepharose™ from Staphylococcus aureus
lyophilized powder
Synonyma:
Protein A–Agarose
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 250 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 7 480,00 Kč | |
| 1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 21 500,00 Kč | |
| 1.5 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 28 100,00 Kč | |
| 5 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 74 000,00 Kč |
O této položce
Form:
lyophilized powder
7 480,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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lyophilized powder
Quality Segment
packaging
glass bottle of 1 g, glass bottle of 1.5 g, glass bottle of 250 mg, glass bottle of 5 g
storage condition
do not freeze
extent of labeling
3 mg per mL
technique(s)
affinity chromatography: suitable
color
white to gray
matrix
Sepharose CL-4B
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥16 mg/mL binding capacity (human IgG)
solubility
water: 1 g/L at 20 °C
suitability
conforms to structure for swelling capacity (1g swells to 4 to 5 mL)
storage temp.
2-8°C
General description
Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.[1]
Application
Protein A-Sepharose was used:
- for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.[2]
- in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.[3]
- as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.[4]
- in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.[5]
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Physical form
Supplied as lyophilized powder stabilized with lactose and dextran.
Preparation Note
Swelling: 1 g swells to approx. 4 ml.
Legal Information
Sepharose is a trademark of Cytiva
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Tato položka | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) - |
| suitability conforms to structure for swelling capacity (1g swells to 4 to 5 mL) | suitability - | suitability - | suitability - |
| form lyophilized powder | form aqueous ethanol suspension | form powder | form cell suspension |
| storage condition do not freeze | storage condition - | storage condition - | storage condition - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility water: 1 g/L at 20 °C | solubility - | solubility H2O: 1 mg/mL, clear, colorless | solubility - |
Skladovací třída
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protokoly
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
Sortimentní položky
Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).
Související obsah
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
Tracie A Hennen-Bierwagen et al.
Plant physiology, 149(3), 1541-1559 (2009-01-27)
Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes
Frédéric Sorgeloos et al.
Journal of virology, 85(7), 3690-3694 (2011-01-14)
The L protein encoded by Theiler's murine encephalomyelitis virus (TMEV) is a unique example of a picornaviral protein encoded by an alternative open reading frame. This protein is an important determinant of TMEV persistence in the mouse central nervous system.
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| P3391-5G | 04061834367819 |
| P3391-250MG | 04061835512874 |
| P3391-1G | 04061835573851 |
| P3391-1.5G | 04061834367802 |
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What can P3391, Protein A-Sepharose® resin tolerate?
1 answer-
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How do I swell P3391, Protein A-Sepharose® resin?
1 answer-
The lyophilized product should be swollen in buffer A for 30 minutes or longer at room temperature. Do not stir with any kind of mechanical stirrer. One gram of powder typically swells to 3-4 mL of the hydrated gel. This, along with other useful information (such as the formulation of buffer A) can be found on the product information sheet (under Documents, above).
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What is the binding capacity of Product P3391, Protein A-Sepharose®, resin for my immunoglobulins?
1 answer-
A table is available on the product information sheet (under Documents, above).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I store the hydrated P3391, Protein A-Sepharose® resin?
1 answer-
Suspensions stored in the refrigerator in the presence of a suitable bacteriostatic agent, such as 20% ETOH, retain IgG binding activity. Another storage option is to put the resin into phosphate buffer saline, pH 7 with about 0.01% preservative (thimerosal or sodium azide).
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How do I clean Product P3391, Protein A-Sepharose®, resin?
1 answer-
It may be possible to clean the resin by washing it with 10-20 volumes of 100 mM Tris or borate buffer, pH8.5, containing 0.5-2.0 M NaCl, followed by 10-20 volumes of 100 mM acetate buffer, pH 4.0, containing0.5-2.0 M NaCl. Re equilibrate the resin with 20 volumes of buffer A.
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