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F4049

ANTI-FLAG^® M2-FITC antibody, Mouse monoclonal

Name: ANTI-FLAG<SUP>®</SUP> M2-FITC antibody, Mouse monoclonal
Brand: SIGMA
Price: 16800 CZK

clone M2, purified immunoglobulin, buffered aqueous solution

Synonyma:

Monoclonal ANTI-FLAG^® M2, Anti-ddddk, Anti-dykddddk

Přihlásit pro zobrazení organizačních a smluvních cen.

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Velikost balení	Dostupnost	Cena
0.2 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	16 800,00 Kč
1 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	40 700,00 Kč
5 x 1 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	56 300,00 Kč

O této položce

NACRES:

NA.32

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

M2, monoclonal

Application:

IF (d)

Citations:

114

16 800,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

Technický servis

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Vlastnosti

biological source

mouse

Quality Level

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Popis

General description

Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.

Application

Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.

Learn more product details in ourFLAG^® application portal.

Physical form

Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Porovnat podobné položky

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1 of 1

Tato položka	A9594	F9291	F3040
Sigma-Aldrich F4049 ANTI-FLAG^® M2-FITC antibody, Mouse monoclonal	Sigma-Aldrich A9594 Monoclonal ANTI-FLAG^® M2-Cy3 antibody, Mouse monoclonal	Sigma-Aldrich F9291 ANTI-FLAG^® BioM2 antibody, Mouse monoclonal	Sigma-Aldrich F3040 Monoclonal ANTI-FLAG^® M1 antibody
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
clone M2, monoclonal	clone M2, monoclonal	clone M2, monoclonal	clone M1, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source (Bioreactor)
conjugate FITC conjugate	conjugate CY3 conjugate	conjugate biotin conjugate	conjugate unconjugated
species reactivity all	species reactivity all	species reactivity all	species reactivity all
form buffered aqueous solution	form buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)	form buffered aqueous glycerol solution	form buffered aqueous solution

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Skladovací třída

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number

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Sortimentní položky

Glycan Labeling

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

Související obsah

Technical Bulletin-F4049

Identification of essential histidine and cysteine residues of the H+/organic cation antiporter multidrug and toxin extrusion (MATE).

Jun-ichi Asaka et al.

Molecular pharmacology, 71(6), 1487-1493 (2007-03-01)

Multidrug and toxin extrusion 1 (MATE1) has been isolated as an H(+)/organic cation antiporter located at the renal brush-border membranes. Previous studies using rat renal brush-border membrane vesicles indicated that cysteine and histidine residues played critical roles in H(+)/organic cation

In vitro expression and analysis of the 826 human G protein-coupled receptors.

Xuechen Lv et al.

Protein & cell, 7(5), 325-337 (2016-04-18)

G protein-coupled receptors (GPCRs) are involved in all human physiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect

Using T. brucei as a biological epitope-display platform to elicit specific antibody responses.

Pete Stavropoulos et al.

Journal of immunological methods, 362(1-2), 190-194 (2010-08-31)

The African trypanosome (Trypanosoma brucei) is transmitted by the bite of the tsetse vector to the mammalian bloodstream where it exists as a completely extracellular parasite. As a result of this exposure, the parasite elicits a robust immune response that

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Globální číslo obchodní položky

Skladová položka	GTIN
F4049-5X1MG	04061833420652
F4049-.2MG	04061833617434
F4049-1MG	04061833617441

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