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F9291

ANTI-FLAG^® BioM2 antibody, Mouse monoclonal

Name: ANTI-FLAG<SUP>®</SUP> BioM2 antibody, Mouse monoclonal
Brand: SIGMA

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonyma:

Monoclonal ANTI-FLAG^® M2, Anti-ddddk, Anti-dykddddk

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Velikost balení	Skladová položka	Dostupnost	Cena

O této položce

NACRES:

NA.32

UNSPSC Code:

12352203

Conjugate:

biotin conjugate

Clone:

M2, monoclonal

Application:

Citations:

128

Informace o stanovených cenách a dostupnosti nejsou v současnosti dostupné.

Technický servis

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Vlastnosti

Quality Level

200

biological source

mouse

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

dot blot: suitable (chemiluminescent detection)

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Popis

General description

The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.

Application

Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.

Learn more product details in our FLAG^® application portal.

Physical form

Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide

Preparation Note

Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tato položka	A9594	F3165	F4042
Sigma-Aldrich F9291 ANTI-FLAG^® BioM2 antibody, Mouse monoclonal	Sigma-Aldrich A9594 Monoclonal ANTI-FLAG^® M2-Cy3 antibody, Mouse monoclonal	Sigma-Aldrich F3165 ANTI-FLAG^® M2 antibody, Mouse monoclonal	Sigma-Aldrich F4042 Monoclonal ANTI-FLAG^® M5 antibody produced in mouse
clone M2, monoclonal	clone M2, monoclonal	clone M2, monoclonal	clone M5, monoclonal
conjugate biotin conjugate	conjugate CY3 conjugate	conjugate unconjugated	conjugate unconjugated
species reactivity all	species reactivity all	species reactivity all	species reactivity all
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin (Purified IgG1 subclass)	antibody form purified immunoglobulin
biological source mouse	biological source mouse	biological source mouse	biological source mouse
technique(s) dot blot: suitable (chemiluminescent detection)	technique(s) direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone	technique(s) western blot: 10 μg/mL (Protein A)	technique(s) western blot (chemiluminescent): 10 μg/mL

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Skladovací třída

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number

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Sortimentní položky

Glycan Labeling

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

Související obsah

Product Information Sheet-F9291

Proteomic Applications of Specialty High Capacity Streptavidin-Coated Multiwell Plates

CD200R1 agonist attenuates mechanisms of chronic disease in a murine model of multiple sclerosis.

Yingru Liu et al.

The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(6), 2025-2038 (2010-02-12)

To assess the effects and mechanisms of a CD200R1 agonist administered during the progressive stage of a multiple sclerosis model, we administered CD200R1 agonist (CD200Fc) or control IgG2a during the chronic phase of disease (days 10-30) in mice with experimental

Real-time single-molecule co-immunoprecipitation analyses reveal cancer-specific Ras signalling dynamics.

Hong-Won Lee et al.

Nature communications, 4, 1505-1505 (2013-02-21)

Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified

RING finger protein 31 promotes p53 degradation in breast cancer cells.

J Zhu et al.

Oncogene, 35(15), 1955-1964 (2015-07-07)

The atypical E3 ubiquitin ligase RNF31 is highly expressed in human breast cancer, the most frequent neoplastic lethality among women. Here, RNF31 depletion in breast cancer cells in combination with global gene expression profiling revealed p53 (TP53) signaling as a

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Globální číslo obchodní položky

Skladová položka	GTIN
F9291-.2MG	04061833621509
F9291-1MG	04061833621516
F9291-5X1MG	04061833420676

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