Benzonase Nuclease is extremely stable. Even with extended incubations at 37 °C, Benzonase® Nuclease maintained > 90% activity for several months.
E8263
Benzonase® Nuclease, ultrapure
≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Benzonase® Nuclease, ultrapure
1 of 1 reviewers received a sample product or took part in a promotion
Synonyma:
Endonuclease from Serratia marcescens
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 5 kU | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 4 640,00 Kč | |
| 25 kU | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 16 100,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Assay:
≥99% (SDS-PAGE)
Biological source:
Serratia marcescens
Recombinant:
expressed in E. coli
Concentration:
≥250 units/μL
4 640,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Serratia marcescens
Quality Level
recombinant
expressed in E. coli
grade
ultrapure grade
assay
≥99% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
30 kDa
concentration
≥250 units/μL
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
Application
The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins.[1] It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.[2]
Ultrapure benzonase nuclease, or Endonuclease from Serratia marcescens, has been used in a study to assess the effect of sodium dodecylbenzenesulfonate and antifoaming agents on the endonuclease activity of Serratia marcescens. Endonuclease from Serratia marcescens has also been used in a study to investigate the structure of chromatin subunits.
Used for the removal of nucleic acid from protein samples.
Biochem/physiol Actions
Benzonase® is a genetically engineered endonuclease from Serratia marcescens.[3][4] The protein is a dimer of 30 kDa subunits with two essential disulfide bonds.[5][6][7][8] This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.
Digests native or heat-denatured DNA and RNA.
Physical form
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
Other Notes
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).
Legal Information
Benzonase® Nuclease is supplied by Merck KGaA, Darmstadt, Germany and/or its affiliates.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Tato položka | |||
|---|---|---|---|
| grade ultrapure grade | grade - | grade - | grade - |
| assay ≥99% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay >99% (SDS-PAGE) | assay ≥99% (SDS-PAGE) |
| biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution |
| application(s) research use | application(s) research use | application(s) research use | application(s) pharma/biopharma processes |
| concentration ≥250 units/μL | concentration ≥250 units/μL | concentration ≥250 units/μL | concentration ≥250 units/μL |
Skladovací třída
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Sortimentní položky
Na této stránce je uvedeno devět často kladených otázek a odpovědí o Benzonase® Nuclease.
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.
Související obsah
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
Syed Nabeel-Shah et al.
Scientific reports, 10(1), 168-168 (2020-01-15)
Chromatin organization influences most aspects of gene expression regulation. The linker histone H1, along with the core histones, is a key component of eukaryotic chromatin. Despite its critical roles in chromatin structure and function and gene regulation, studies regarding the
Laure Teysset et al.
Journal of virology, 77(9), 5451-5463 (2003-04-15)
The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. Although these proteins are known to be necessary for protein processing, reverse transcription, and integration, respectively, the function of
Hung Ho-Xuan et al.
Nucleic acids research, 48(18), 10368-10382 (2020-09-22)
Circular RNAs (circRNAs) encompass a widespread and conserved class of RNAs, which are generated by back-splicing of downstream 5' to upstream 3' splice sites. CircRNAs are tissue-specific and have been implicated in diseases including cancer. They can function as sponges
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| E8263-25KU | 04061833608685 |
| E8263-5KU | 04061833608692 |
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Hello, I purchased it recently while the mailroom didn't notify me immediately when it arrived, so it stayed in 4 degree instead or -20 for around two days. Could you please provide me some information if it will highly affect the efficiency of the enzyme
1 answer-
Helpful?
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Does this benzonase contain any epitope tag?
1 answer-
Product E8263 does not have any epitope tag.
Helpful?
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