AMPD1
DNase I
Amplification Grade
Synonyma:
Deoxyribonuclease I
About This Item
Doporučené produkty
form
liquid
concentration
1 unit/μL
technique(s)
RT-PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
Související kategorie
General description
DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided, DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription.
No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laboratory comparisons have shown that Sigma′s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers.
Application
- for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase.
- to hydrolyze extracellular matrix (ECM) components and enhance photosensitizer penetration into the biofilm to determine the efficacy of antimicrobial photodynamic therapy (aPDT) on Candida albicans biofilms
- to remove contaminating DNA from total RNA extracted from cattle blood samples
Features and Benefits
- Suitable for the elimination of DNA from RNA
- Minimal RNase activity available
- Optimized 10× reaction buffer and Stop Solution for complete inactivation of DNase I
Suitability
Unit Definition
Legal Information
inhibitor
related product
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Zákazníci si také prohlíželi
Sortimentní položky
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
Protokoly
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Související obsah
The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.
Polymerázová řetězová reakce (PCR) je technika pro amplifikaci molekul nukleových kyselin a běžně se používá v mnoha aplikacích, včetně RT-PCR, PCR s horkým startem, PCR s koncovým bodem a dalších.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis.