10104159001
Roche
DNase I
grade II, from bovine pancreas
Synonyma:
Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Vybrat velikost
100 MG
3 500,00 Kč
3 500,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Vybrat velikost
Změnit zobrazení
100 MG
3 500,00 Kč
About This Item
UNSPSC Code:
12352204
3 500,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Doporučené produkty
biological source
bovine pancreas
Quality Level
form
lyophilized
packaging
pkg of 100 mg
manufacturer/tradename
Roche
technique(s)
DNA extraction: suitable
optimum pH
~7.0
storage temp.
2-8°C
Související kategorie
General description
Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1] DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.[2]
Application
Unit Definition
One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.
Preparation Note
Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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