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A2383

Sigma-Aldrich

Adenosine 5′-triphosphate disodium salt hydrate

Adenosine 5′-triphosphate disodium salt hydrate

Grade I, ≥99%, from microbial

Synonyma:

ATP disodium salt

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen

Vybrat velikost

1 G
1 900,00 Kč
5 G
6 240,00 Kč
10 G
6 760,00 Kč
25 G
16 700,00 Kč

1 900,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Všechny fotografie(6)

Vybrat velikost

Změnit zobrazení
1 G
1 900,00 Kč
5 G
6 240,00 Kč
10 G
6 760,00 Kč
25 G
16 700,00 Kč

About This Item

Empirický vzorec (Hillův zápis):
C10H14N5Na2O13P3 · xH2O
Číslo CAS:
34369-07-8
Molekulová hmotnost:
551.14 (anhydrous basis)
EC Number:
213-579-1
MDL number:
MFCD00150755
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
24890481
NACRES:
NA.51

1 900,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

biological source

microbial

Quality Level

200

type

Grade I

assay

≥99%

form

powder

solubility

H2O: 50 mg/mL

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1

InChI key

NTBQNWBHIXNPRU-MSQVLRTGSA-L

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Application

Adenosine 5′-triphosphate disodium salt hydrate has been used:
  • to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures[1]
  • bone marrow-derived macrophages (BMDM) treatment[2]
  • as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)[3]

Biochem/physiol Actions

Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo.[4] ATP is used in many cellular processes, respiration, biosynthetic reactions, active transport, and motility.[5] ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase that produce the secondary messenger cyclic adenosine monophosphate (cAMP).[6] ATP provides the metabolic energy to drive metabolic pumps.[5] It serves as a coenzyme in a wide array of enzymatic reactions.[7]
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems
P2 purinocepter agonist.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)

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Osvědčení o analýze (COA)

Lot/Batch Number
0000425728
0000425727
0000425727
0000425728
0000424421

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Navštívit knihovnu dokumentů

J G Donaldson et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(14), 6408-6412 (1992-07-15)
The coatomer is a cytosolic protein complex that reversibly associates with Golgi membranes and is implicated in modulating Golgi membrane transport. The association of beta-COP, a component of coatomer, with Golgi membranes is enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S]), a
Release of extracellular ATP by bacteria during growth
Mempin R, et al.
BMC Microbiology, 13(1), 301-301 (2013)
Rui Nian et al.
Biotechnology progress, 25(4), 1078-1085 (2009-06-25)
The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the
Physiology, Adenosine Triphosphate (ATP)
NCBI Bookshelf null
Mass spectrometry and NMR spectroscopy based quantitative metabolomics
Proteomics. Clinical Applications (2020)
Zobrazit všechny související dokumenty

Protokoly

Enzymatic Assay & ATP Sensitivity Test of Luciferase

Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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Questions

  1. Can I dissolve this ATP in water and store it?

    1 answer

    1. This item can be solubilized in water at 50 mg/mL yielding a clear, colorless solution. Aqueous solutions of ATP are stable for months at -15C and or one week when stored at -0C as mentioned in the Product Information Sheet.

      For more information, please navigate to the ‘DOCUMENTATION’ section to access the Product Information Sheet under 'More Documents':
      https://www.sigmaaldrich.com/product/sigma/a2383#product-documentation

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  1. 1 Ratings-Only Review

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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