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A2383

Adenosine 5′-triphosphate disodium salt hydrate

≥99%, microbial, powder

Adenosine 5′-triphosphate disodium salt hydrate

Synonyma:

ATP disodium salt

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Velikost baleníSkladová položkaDostupnostCena
1 g
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1 980,00 Kč
5 g
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6 490,00 Kč
10 g
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7 030,00 Kč
25 g
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17 100,00 Kč

O této položce

Empirický vzorec (Hillův zápis):
C10H14N5Na2O13P3 · xH2O
Číslo CAS:
Molekulová hmotnost:
551.14 (anhydrous basis)
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.51
EC Number:
213-579-1
MDL number:
Assay:
≥99%
Biological source:
microbial
Form:
powder
Solubility:
H2O: 50 mg/mL
Storage temp.:
−20°C

1 980,00 Kč


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Název produktu

Adenosine 5′-triphosphate disodium salt hydrate, Grade I, ≥99%, from microbial

biological source

microbial

Quality Level

type

Grade I

assay

≥99%

form

powder

solubility

H2O: 50 mg/mL

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1

InChI key

NTBQNWBHIXNPRU-MSQVLRTGSA-L

Application

Adenosine 5′-triphosphate disodium salt hydrate has been used:
  • to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures
  • bone marrow-derived macrophages (BMDM) treatment[1]
  • as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)[2]

Biochem/physiol Actions

Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo.[3] ATP is used in many cellular processes, respiration, biosynthetic reactions, active transport, and motility.[4] ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase that produce the secondary messenger cyclic adenosine monophosphate (cAMP). ATP provides the metabolic energy to drive metabolic pumps.[4] It serves as a coenzyme in a wide array of enzymatic reactions.
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems
P2 purinocepter agonist.

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Tato položka
A3377A7699A1852
biological source

microbial

biological source

microbial

biological source

microbial

biological source

microbial (Corynebacterium sp.)

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

powder

form

crystalline

form

powder

form

powder

assay

≥99%

assay

≥97% (HPLC)

assay

≥99% (HPLC)

assay

≥99% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: 50 mg/mL

solubility

H2O: 50 mg/mL

solubility

H2O: 50 mg/mL, clear, colorless

solubility

water: 50 mg/mL, clear, colorless


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Skladovací třída

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)



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Osvědčení o analýze (COA)

Lot/Batch Number

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Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

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Protokoly

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.

Související obsah

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

Product Information Sheet


Physiology, Adenosine Triphosphate (ATP)
NCBI Bookshelf null
Mass spectrometry and NMR spectroscopy based quantitative metabolomics
Proteomics. Clinical Applications (2020)
J G Donaldson et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(14), 6408-6412 (1992-07-15)
The coatomer is a cytosolic protein complex that reversibly associates with Golgi membranes and is implicated in modulating Golgi membrane transport. The association of beta-COP, a component of coatomer, with Golgi membranes is enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S]), a



Globální číslo obchodní položky

Skladová položkaGTIN
A2383-25G04061833350065
A2383-10G04061835553228
A2383-5G04061835553235
A2383-1G04061835515271

Questions

1–3 of 3 Questions  
  1. I would like to know how many g of ATP disodium salt hydrate contains in FLAAS -1vol vial? Thanks

    1 answer
    1. In catalog number FLAAS, the specification for our vial Content is 0.90 - 1.10 mg ATP/vial by Enzymatic Assay.  Please refer to the specific lot certificate of analysis for an individual quantity.  

      Helpful?

  2. What volume of water should 1mg ATP be reconstituted in & what would be its corresponding concentration?

    1 answer
    1. This product is soluble in water up to 50 mg/mL. The appropriate stock concentration for assays can be determined by the end user. The molecular weight is 551.14 (anhydrous basis), and the water content can be found on the lot-specific certificate of analysis. Using the molecular weight, the water content, and the chosen concentration for the ATP solution, the molarity can be calculated.

      Helpful?

  3. Can I dissolve this ATP in water and store it?

    1 answer
    1. This item can be solubilized in water at 50 mg/mL yielding a clear, colorless solution. Aqueous solutions of ATP are stable for months at -15C and or one week when stored at -0C as mentioned in the Product Information Sheet.

      For more information, please navigate to the ‘DOCUMENTATION’ section to access the Product Information Sheet under 'More Documents':
      https://www.sigmaaldrich.com/product/sigma/a2383#product-documentation

      Helpful?

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