A3643

Agarose

Type IV, Special High EEO

• Synonyma: 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
• Číslo CAS: 9012-36-6
• UNSPSC Code: 41105317
• PubChem Substance ID: 24890793
• EC Number: 232-731-8
• NACRES: NA.21
• MDL number: MFCD00081294

Vlastnosti

• biological source: algae (marine)
• Quality Level: 200
• type: Type IV
• form: powder
• technique(s): electrophoresis: suitable
• EEO: ≥0.3
• SMILES string: O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Popis

Application

Agarose has been used in the electrophoresis of RNA for northern blot. It has also been used in coating microscope slides for counter immunoelectrophoresis.

Analysis Note

Characterized by lower sulfate content and lower non-specific protein binding capacity than Type III. Trailing and smearing of proteins due to non-specific protein binding is minimized.

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)