A5304

Agarose

Special High EEO

• Synonyma: 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
• Číslo CAS: 9012-36-6
• EC Number: 232-731-8
• MDL number: MFCD00081294
• UNSPSC Code: 41105317
• PubChem Substance ID: 24890968
• NACRES: NA.28

Vlastnosti

• form: powder
• technique(s): electrophoresis: suitable; immunoelectrophoresis: suitable
• impurities: ≤11% moisture content
• ash: ≤1.5%
• turbidity: ≤4 NTU (1.5% gel)
• EEO: ≤0.40
• transition temp: gel point 36 °C ±1.5 °C (1.5% gel)
• gel strength: ≥700 g/cm² (1% gel)
• anion traces: sulfate (SO₄^2-): ≤0.2%
• storage temp.: room temp
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Popis

Suitability

Suitable for counterimmunoelectrophoresis and immunoelectrophoretic techniques with significant cathodal migration.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

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Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)