The concentration is 50 µg/ml in PBS, pH 7.4.
537060
Propidium Iodide Solution
A convenient form of propidium iodide useful for flow cytometry studies.
Synonyma:
Propidium Iodide Solution
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 5 mL | K dispozici k odeslání DNESodAreál Kühne+Nagel spol. s r.o. | 3 470,00 Kč |
O této položce
Empirický vzorec (Hillův zápis):
C27H34I2N4
Číslo CAS:
Molekulová hmotnost:
668.39
UNSPSC Code:
12352200
NACRES:
NA.47
MDL number:
Technický servis
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Dovolte nám, abychom vám pomohliQuality Level
description
RTECS - SF7949600
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
input
sample type intact cells
fluorescence
λex 536 nm, λem 617 nm
shipped in
ambient
storage temp.
2-8°C
SMILES string
[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
General description
Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Physical form
50 µg/ml in PBS, pH 7.4.
Preparation Note
Following initial use, aliquot and freeze (-20°C).
Other Notes
Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Tato položka | |||
|---|---|---|---|
| form liquid | form solid | form liquid | form powder |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| storage condition OK to freeze, protect from light | storage condition OK to freeze, protect from light | storage condition - | storage condition - |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
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Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protokoly
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
Související obsah
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| 537060-5ML | 04055977195927 |
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what is the concentration of the PI solution
1 answer-
Helpful?
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What is the recommended protocol for use/ working concentration?
1 answer-
The working concentration must be determined by the end user.
In the following reference: Cytometry. 1994 Sep 1;17(1):59-65. PMID: 7528124 DOI: 10.1002/cyto.990170108; 5ug/ML of PI was directly added in the culture medium for 30min. at 37C.Helpful?
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