The solution stability of this specific product has not been determined, but solutions are quite stable. Product P4864, a 1 mg/mL prepared solution, is stored refrigerated and offers a warranty of 1 year from the date of shipment.
P4170
Propidium iodide
≥94.0% (HPLC), fluorescent dye, powder
Synonyma:
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
10 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 1 920,00 Kč |
25 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 3 290,00 Kč |
100 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 5 240,00 Kč |
250 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 11 200,00 Kč |
500 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 20 700,00 Kč |
1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 43 900,00 Kč |
O této položce
Empirický vzorec (Hillův zápis):
C27H34I2N4
Číslo CAS:
Molekulová hmotnost:
668.39
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
41106305
EC Number:
247-081-0
MDL number:
Beilstein/REAXYS Number:
3843838
Assay:
≥94.0% (HPLC)
Form:
powder
1 920,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliNázev produktu
Propidium iodide, ≥94.0% (HPLC)
assay
≥94.0% (HPLC)
form
powder
mp
220-225 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
General description
Application
Features and Benefits
This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
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Tato položka | |||
|---|---|---|---|
| assay ≥94.0% (HPLC) | assay - | assay - | assay ≥94% (HPLC) |
| form powder | form solid | form liquid | form powder |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mp 220-225 °C (dec.) (lit.) | mp - | mp - | mp 220-225 °C (dec.) (lit.) |
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signalword
Warning
hcodes
Hazard Classifications
Muta. 2
Skladovací třída
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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How long can we stored PI dissolved in water?
1 answer-
Helpful?
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Is it necessary to carry out an RNase treatment before staining with propidium iodide in my immunofluorescence assay on insect tissue fixed in 4% paraformaldehyde? Additionally, is there the complete staining protocol for propidium iodide in immunofluorescence using a confocal microscope?
1 answer-
The product has not been validated for immunofluorescence assay in our laboratory to establish a reference protocol. In cases where propidium iodide binds to both DNA and RNA, the use of RNase is common to differentiate DNA staining from undesired RNA staining. For an application protocol, one may refer to a citation of this product in an immunofluorescence assay that utilizes RNase to visualize DNA-specific staining. The citation is as follows: J Cancer. 2020 Mar 5;11(11):3274-3287. The article discusses the dual role of BI 2536, a small-molecule inhibitor targeting PLK1, in inducing apoptosis and attenuating autophagy in neuroblastoma cells. The PMID for the article is 32231733.
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What is the procedure for dissolving this product?
1 answer-
Propidium iodide is soluble in water at a concentration of 1 mg/mL.
Helpful?
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Is this reagent suited for viability assays at FACS?
1 answer-
While this product is not tested for any specific application, propidium iodide is commonly used in cell-based assays such as FACS. Please see the link below to review publications detailing the use of this item in flow cytometry:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22p4170%22+flow+cytometry&btnG=Helpful?
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What is the method to dissolve this product?
1 answer-
Propidium iodide can be dissolved in water at a concentration of 1 mg/mL. Please see the sample Certificate of Analysis for solubility information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4170-BULKWXBF1544V.pdf
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