For product 05-636, a good buffer to use is the supplied buffer: 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. This is both the storage and recommended working buffer for this antibody.
05-636
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
clone JBW301, Upstate®, from mouse
Synonyma:
H2AXS139P, Histone H2A.X (phospho S139)
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 25 μg | Očekávané datum odeslání11. května 2026odAreál Kühne+Nagel spol. s r.o. | 4 910,00 Kč | |
| 200 μg | Očekávané datum odeslání11. května 2026odAreál Kühne+Nagel spol. s r.o. | 18 400,00 Kč |
O této položce
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
JBW301, monoclonal
Species reactivity:
vertebrates
Application:
ChIP, ICC, IF, IHC, WB
Citations:
3207
4 910,00 Kč
Očekávané datum odeslání11. května 2026podrobné informace
Technický servis
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Dovolte nám, abychom vám pomohlibiological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
clone
JBW301, monoclonal
species reactivity
vertebrates
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer139)
General description
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
17 kDa
Immunogen
peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X
Application
Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Anti-phospho-Histone H2A.X (Ser139) Antibody clone JBW301, recognizes Histone H2A.X phosphorylated at Ser139.
Physical form
Format: Purified
Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
Protein G Purified
Preparation Note
Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Analysis Note
Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE205
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
Tato položka | |||
|---|---|---|---|
| species reactivity vertebrates | species reactivity human | species reactivity human, rat, mouse | species reactivity human |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone JBW301, monoclonal | clone JBW301, monoclonal | clone JBW301, monoclonal | clone JBW301, monoclonal |
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified antibody |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| technique(s) ChIP: suitable, immunofluorescence: suitable, western blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable | technique(s) ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable |
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Skladovací třída
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sortimentní položky
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Zjistěte, jaké jsou rozdíly mezi monoklonálními a polyklonálními protilátkami, včetně způsobu generování protilátek, počtu klonů a formátů protilátek.
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"Recognizing both the tremendous opportunities and the challenges facing cancer research, we are dedicated to developing and refining tools and technologies for the study of cancer. With our comprehensive portfolio, including the Upstate®, Chemicon®, and Calbiochem® brands of reagents and antibodies, researchers can count on dependable, high quality solutions for analyzing all the hallmarks of cancer."
Cancer is a complex disease manifestation. At its core, it remains a disease of abnormal cellular proliferation and inappropriate gene expression. In the early days, carcinogenesis was viewed simply as resulting from a collection of genetic mutations that altered the gene expression of key oncogenic genes or tumor suppressor genes leading to uncontrolled growth and disease (Virani, S et al 2012). Today, however, research is showing that carcinogenesis results from the successive accumulation of heritable genetic and epigenetic changes. Moreover, the success in how we predict, treat and overcome cancer will likely involve not only understanding the consequences of direct genetic changes that can cause cancer, but also how the epigenetic and environmental changes cause cancer (Johnson C et al 2015; Waldmann T et al 2013). Epigenetics is the study of heritable gene expression as it relates to changes in DNA structure that are not tied to changes in DNA sequence but, instead, are tied to how the nucleic acid material is read or processed via the myriad of protein-protein, protein-nucleic acid, and nucleic acid-nucleic acid interactions that ultimately manifest themselves into a specific expression phenotype (Ngai SC et al 2012, Johnson C et al 2015). This review will discuss some of the principal aspects of epigenetic research and how they relate to our current understanding of carcinogenesis. Because epigenetics affects phenotype and changes in epigenetics are thought to be key to environmental adaptability and thus may in fact be reversed or manipulated, understanding the integration of experimental and epidemiologic science surrounding cancer and its many manifestations should lead to more effective cancer prognostics as well as treatments (Virani S et al 2012).
Effect of Ku80 deficiency on mutation frequencies and spectra at a LacZ reporter locus in mouse tissues and cells.
Busuttil, RA; Mu?oz, DP; Garcia, AM; Rodier, F; Kim, WH; Suh, Y; Hasty, P; Campisi, J; Vijg, J
Testing null
Unsuitability of lymphoblastoid cell lines as surrogate of cryopreserved isolated lymphocytes for the analysis of DNA double-strand break repair activity.
Zijno, Andrea, et al.
Mutation Research, 684, 98-105 (2010)
Irene V Bijnsdorp et al.
Cancer science, 101(2), 440-447 (2009-11-06)
The pyrimidine trifluorothymidine (TFT) inhibits thymidylate synthase (TS) and can be incorporated into the DNA. TFT, as part of TAS-102, is clinically evaluated in phase II studies as an oral chemotherapeutic agent. Erlotinib is a tyrosine kinase inhibitor of the
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hi. The antiboy is in powdered form. Please guide which buffer is best to reconstitute the antibody and the amount of buffer to be used for reconstitution.
1 answer-
Helpful?
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この抗体は何に溶けていますか？
1 answer-
This product is in 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
Helpful?
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Your product quoted in https://doi.org/10.1016/j.mrgentox.2010.05.009 as 1:1000 dilution.if i use 05-636-25UG, how much µg/ml antibody shall I have to use for whole cell ELISA.
1 answer-
For 05-636, we have not studied this internally for ELISA and therefore, do not have suggestions on using this for ELISA.
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