T7660

Tetracycline hydrochloride

powder, BioReagent, suitable for cell culture

• Synonyma: Tetracycline HCL
• Empirický vzorec (Hillův zápis): C₂₂H₂₄N₂O₈ · HCl
• Číslo CAS: 64-75-5
• Molekulová hmotnost: 480.90
• UNSPSC Code: 51284043
• NACRES: NA.76
• PubChem Substance ID: 24900468
• EC Number: 200-593-8
• Beilstein/REAXYS Number: 3844873
• MDL number: MFCD00078142

Vlastnosti

• product line: BioReagent
• Quality Segment: 200
• form: powder
• storage condition: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: yellow
• mp: 220-223 °C (lit.)
• antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria
• mode of action: protein synthesis | interferes
• storage temp.: −20°C
• SMILES string: Cl.CN(C)[C@H]1[C@@H]2C[C@H]3C(=C(O)[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c4c(O)cccc4[C@@]3(C)O
• InChI: 1S/C22H24N2O8.ClH/c1-21(31)8-5-4-6-11(25)12(8)16(26)13-9(21)7-10-15(24(2)3)17(27)14(20(23)30)19(29)22(10,32)18(13)28;/h4-6,9-10,15,25,27-28,31-32H,7H2,1-3H3,(H2,23,30);1H/t9-,10-,15-,21+,22-;/m0./s1
• InChI key: XMEVHPAGJVLHIG-FMZCEJRJSA-N

Popis

General description

Chemical structure: tetracycline

Application

Tetracycline is a broad spectrum polyketide antibiotic with clinical uses in treating bacterial infections such as Rocky Mountain spotted fever, typush fever, tick fevers, Q fever, and Brill-Zinsser disease and to treat upper respiratory infections and acne. It has been used in studies of multidrug resistance and potential side effects including acute pancreatitis. It is recommended for use in cell culture applications at 10 mg/L.

Biochem/physiol Actions

Mode of Action: Tetracycline passively diffuses through proin channels in the cell membrane, binding to 30S ribosomes and inhibits protein synthesis by preventing access of aminoacyl tRNA to the acceptor site on the mRNA-ribosome complex. It also binds to the bacterial 50S ribosomal subunit, altering the membrane and causing intracellular components to leak from bacterial cells. The inhibitory effects can be reversed by washing, suggesting that it is the reversibly bound antibiotic, and not the irreversibly bound drug, that is responsible for antibacterial action.

Mode of Resistance: The effects are inactivated via a loss of cell wall permeability.

Antimicrobial spectrum: Includes a wide range of antimicrobial activity against gram-positive and gram-negative bacteria.

Preparation Note

The product is freely soluble in water, slightly soluble in 96% ethanol, and is practically insoluble in acetone. Tetracycline is rapidly destroyed by alkali hydroxide solutions and standing water solutions become turbid due to hydrolysis and precipitation. The potency of tetracycline is reduced in solutions with pH below 2.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place

Disclaimer

This product should be frozen below 0°C and protected from light and moisture. In these conditions, the product has been shown to retain activity for 4 years. Stock solutions should be stored at -20°C and are stable at 37°C for 4 days.

Bezpečnostní informace

• pictograms: GHS08,GHS07,GHS09
• signalword: Warning
• hcodes: H315,H319,H335,H361fd,H410
• pcodes: P202 - P261 - P273 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves