SHC216
MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA
Targets no known genes from any species
Synonyma:
MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
Přihlásit pro zobrazení organizačních a smluvních cen.
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Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 1 ea | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 9 870,00 Kč |
O této položce
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
9 870,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliQuality Level
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
General description
The MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones.[1] This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Tato položka | |||
|---|---|---|---|
| concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Skladovací třída
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Související obsah
Instructions
Cancer therapies activate RIG-I-like receptor pathway through endogenous non-coding RNAs.
Ranoa DR
Oncotarget, 7(18) (2016)
Jing Pu et al.
The Journal of cell biology, 216(12), 4183-4197 (2017-10-11)
Lysosomes play key roles in the cellular response to amino acid availability. Depletion of amino acids from the medium turns off a signaling pathway involving the Ragulator complex and the Rag guanosine triphosphatases (GTPases), causing release of the inactive mammalian
Deanna M Santer et al.
Journal of immunological methods, 445, 15-22 (2017-03-10)
Type III interferons (IFN-lambdas) are important antiviral cytokines that also modulate immune responses acting through a unique IFN-λR1/IL-10R2 heterodimeric receptor. Conflicting data has been reported for which cells express the IFN-λR1 subunit and directly respond to IFN-λs. In this study
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| SHC216-1EA | 04061826600627 |