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S6639

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

SSC Buffer 20× Concentrate

Synonyma:

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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Velikost baleníSkladová položkaDostupnostCena
1 L
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3 320,00 Kč

O této položce

NACRES:
NA.25
UNSPSC Code:
41105319

3 320,00 Kč


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grade

Molecular Biology

Quality Segment

description

Saline-Sodium Citrate buffer, made with ultrapure water

sterility

0.2 μm filtered

form

solution

color

colorless

pH

6.9 —7.1

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence[1]
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA[2]
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Preparation Note

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

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Tato položka
SRE0068S201593017
grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

Molecular Biology

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

solution

form

liquid

form

solution

form

solution

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

pH

6.9 —7.1

pH

-

pH

-

pH

7.0±0.2


Skladovací třída

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protokoly

Naučte se základy Northern a Southern blottingu s protokoly a aplikacemi pro přenos makromolekul na membránové nosiče.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Sortimentní položky

Dostupné postupy, činidla a vybavení pro fluorescenční in situ hybridizaci (FISH).

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.






Globální číslo obchodní položky

Skladová položkaGTIN
S6639-1L04061838258816

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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