Percoll is produced by Cytiva, formerly known as GE Life Sciences. Details regarding the proprietary manufacturing process are not publicly available. However, it can be mentioned that the Percoll particles range in size from 15 to 30 nanometers. While the density of Percoll solution is measured, the specific information regarding the amount of coating on the silica particles is not provided.
P1644
Percoll®
pH 8.5-9.5 (20 °C)
Synonyma:
Percoll Density Gradient Media
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| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|
O této položce
Informace o stanovených cenách a dostupnosti nejsou v současnosti dostupné.
Technický servis
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aseptically filled
Quality Level
form
colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
technique(s)
cell culture | mammalian: suitable
color
clear colorless to faint yellow
pH
8.5-9.5 (20 °C)
application(s)
hematology
histology
storage temp.
2-8°C
General description
Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.
Application
Percoll® has been used in sperm preparation to study the energy requirements of bovine embryos.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Legal Information
Percoll is a registered trademark of Cytiva
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Tato položka | |||
|---|---|---|---|
| sterility aseptically filled | sterility aseptically filled | sterility aseptically filled | sterility aseptically filled |
| form colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) ) | form colloidal | form colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) | form solution |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | plant: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| application(s) hematology | application(s) - | application(s) agriculture | application(s) - |
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Skladovací třída
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Protokoly
Pro přípravu Percollova gradientu je třeba osmolalitu Percollu upravit fyziologickým roztokem nebo médiem pro buněčné kultury, aby byl Percoll izotonický s fyziologickými roztoky solí.
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Sortimentní položky
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Související obsah
Development of bovine embryos in vitro as affected by energy substrates.
Rosenkrans Jr C F, et al.
Biology of Reproduction, 49(3), 459-462 (1993)
Noa Bossel Ben-Moshe et al.
Nature communications, 10(1), 3266-3266 (2019-07-25)
Complex interactions between different host immune cell types can determine the outcome of pathogen infections. Advances in single cell RNA-sequencing (scRNA-seq) allow probing of these immune interactions, such as cell-type compositions, which are then interpreted by deconvolution algorithms using bulk
R Hovius et al.
Biochimica et biophysica acta, 1021(2), 217-226 (1990-01-29)
Rat liver mitochondria were isolated by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation, as assessed by marker enzyme analysis, latency of cytochrome-c oxidase, respiratory control index and electron microscopy. Two different
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| P1644-25ML | 04061838262059 |
| P1644-500ML | 04061838262066 |
| P1644-1L | 04061838262042 |
| P1644-100ML | 04061838262035 |
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What is the details about the manufacturing process of Percoll, specifically regarding the amount of PVP used during bead soaking or the approximate PVP concentration within Percoll?
1 answer-
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What is procedure for sperm preparation for ivf with percoll gradient
1 answer-
Please note that this product is intended for research use only. This material is not tested for the isolation of sperm samples. The following reference may be helpful:
Chen. Y. et al., Int. J. Fertil., 37, 315-319 (1992).Please see the link below to review the product datasheet for additional information.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdfHelpful?
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CAN I FREEZE PERCOLL (P1644)? I SEE IN OTHER REF THAT I CAN, BUT NOT IN THIS REF
1 answer-
This material should not be stored frozen for long periods of time. As per the product information sheet, if stored at –20 °C, gradients form upon thawing, necessitating mixing the contents of the bottle before use. Please see the link below to review the product datasheet for further information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfHelpful?
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Percoll (P1644) 을 gradient 만드는데 사용했는데요, P4937도 같은 용도로 사용 가능한가요?
1 answer-
Yes, product P4937 can be used for the same use as product P1644.
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I often find crystals. Can I filter the solution before use? What filter size could I use without affecting the functionality of the product?
1 answer-
Particles may be removed by filtration or low speed centrifugation. More information about the particles filtration can be found in the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfHelpful?
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Is there a "best" salt solution to use with Product P1644, Percoll®?
1 answer-
Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested. Sucrose is included to prevent aggregation. You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Percoll®, Product P1644, be autoclaved?
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Percoll can be autoclaved, but this must be done without any salts or sucrose present.
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What is the difference between Percoll® Products P4937 and P1644?
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Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.
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Can you help me calculate gradients for Percoll®, Product P1644?
1 answer-
The product information sheet (under Documents, above) has an equation for calculating density of a given solution.
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