MAK552

Fluo-4 No Wash Calcium Assay Kit

sufficient for fluorometric test plates

• NACRES: NA.84
• UNSPSC Code: 41116104

Vlastnosti

• detection method: fluorometric
• relevant disease(s): endocrinological disorders, diabetes; genitourinary disorders; neurological disorders; cancer
• storage temp.: −20°C

Popis

General description

The Fluo-4 No Wash Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are preloaded with Fluo-4 AM which can cross the cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-4 AM are cleaved by non-specific cell esterase, resulting in a negatively charged Fluo-4 dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with screening compounds, the receptor signals the release of intracellular calcium, which greatly increases the fluorescence of Fluo-4.

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• wgk: WGK 3