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M8537

MCDB 131 Medium

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonyma:

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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Velikost baleníSkladová položkaDostupnostCena
1 L
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1 410,00 Kč
10 L
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5 000,00 Kč
10 x 1 L
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5 670,00 Kč

O této položce

UNSPSC Code:
12352207
NACRES:
NA.75

1 410,00 Kč


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Název produktu

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

concentration

11.6 g/L

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

General description

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Application

MCDB-131 Medium has been used:

  • as a component of the basal medium to culture bovine retinal endothelial cells[1]
  • to culture bovine lung microvascular endothelial cells[2]
  • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells[3]

Preparation Note

Formulated to contain 11.6 grams of powder per liter of medium.
Supplement with 1.18 g/L sodium bicarbonate.

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Tato položka
M7403M6395M6770
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

powder

form

powder

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

concentration

11.6 g/L

concentration

-

concentration

-

concentration

-


Skladovací třída

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Lot/Batch Number

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Protokoly

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Související obsah


Abdul Jaleel et al.
Molecular and cellular biochemistry, 451(1-2), 1-10 (2018-06-24)
Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally
Kathleen M Job et al.
Anesthesia and analgesia, 123(2), 382-393 (2016-06-23)
The endothelial glycocalyx is an important component of the vascular permeability barrier, forming a scaffold that allows serum proteins to create a gel-like layer on the endothelial surface and transmitting mechanosensing and mechanotransduction information that influences permeability. During acute inflammation
G S Ajithkumar et al.
Cardiovascular toxicology, 16(4), 390-405 (2015-12-15)
Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival



Globální číslo obchodní položky

Skladová položkaGTIN
M8537-10L04061834063285
M8537-1L04061834063308
M8537-10X1L04061834063292

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