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I2002

Sigma-Aldrich

Isocitric Dehydrogenase (NADP) from porcine heart

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

Synonyma:

threo-Ds-Isocitrate: NADP+ oxidoreductase, ICDH, IDH

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About This Item

Číslo CAS:
9028-48-2
Číslo enzymu podle klasifikace EK:
1.1.1.42 (BRENDA, IUBMB)
EC Number:
200-289-5
MDL number:
MFCD00131420
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type IV

form

buffered aqueous glycerol solution

quality

≤ 0.2% of main activity is obtained with NAD as cofactor.

specific activity

3-20 units/mg protein

composition

Protein biuret

shipped in

wet ice

storage temp.

2-8°C

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Application

Isocitric dehydrogenase (IDH) has been used:
  • for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
  • as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
  • in isocitrate assay

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH1, IDH2, and IDH3.

Unit Definition

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

inhibitor

Č. produktu
Popis
Stanovení ceny

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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