This product is tested for the expansion of TF-1 cells. The activity is reported as the ED50 in nanograms per milliliter. The activity in International Units has not been established. The ED50 value is reported on the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/h5166#product-documentation
H5166
Erythropoietin human
EPO, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonyma:
EPO
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Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 10 μg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 7 080,00 Kč |
O této položce
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
dimer 36 kDa (glycosylated)
Impurities:
≤1 EU/μg
7 080,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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human
Quality Level
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤5.0 ng/mL ED50
quality
endotoxin tested
mol wt
dimer 36 kDa (glycosylated)
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... EPO(2056)
General description
EPO has been cloned from various species including human, murine, canine, and others. The mature proteins from the various species are highly conserved and exhibit greater than 80% amino acid sequence identity. EPO contains three N-linked glycosylation sites. The glycosylation of erythropoietin is required for the biological activities of erythropoietin in vivo.
Biochem/physiol Actions
Erythropoietin (EPO), produced primarily by the kidney, is the primary regulatory factor of erythropoiesis. It promotes the proliferation, differentiation, and survival of the erythroid progenitors. Erythropoietin stimulates erythropoiesis by inducing growth and differentiation of burst forming units and colony forming units into mature red blood cells. EPO produced by kidney cells is increased in response to hypoxia or anemia. The biological effects of erythropoietin are mediated by the erythropoietin receptor, which binds EPO with high affinity and is a potent EPO antagonist.
Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation.
Preparation Note
Human EPO is expressed as a glycosylated 36 kDa monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
1 of 1
Tato položka | |||
|---|---|---|---|
| assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| biological source human | biological source mouse | biological source human | biological source human |
| recombinant expressed in HEK 293 cells | recombinant expressed in NSO cells | recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 100 | Quality Level 200 | Quality Level 300 | Quality Level - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized |
Skladovací třída
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protokoly
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Sortimentní položky
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Související obsah
Instructions
Mawadda Alnaeeli et al.
Diabetes, 63(7), 2415-2431 (2014-03-22)
Obesity-induced white adipose tissue (WAT) inflammation and insulin resistance are associated with macrophage (Mф) infiltration and phenotypic shift from "anti-inflammatory" M2-like to predominantly "proinflammatory" M1-like cells. Erythropoietin (EPO), a glycoprotein hormone indispensable for erythropoiesis, has biological activities that extend to
Lamia Lamrani et al.
Blood, 124(7), 1136-1145 (2014-06-22)
Thrombosis is common in patients suffering from myeloproliferative neoplasm (MPN), whereas bleeding is less frequent. JAK2(V617F), the main mutation involved in MPN, is considered as a risk factor for thrombosis, although the direct link between the mutation and hemostatic disorders
Cynthia So-Osman et al.
Anesthesiology, 120(4), 852-860 (2014-01-18)
Patient blood management is introduced as a new concept that involves the combined use of transfusion alternatives. In elective adult total hip- or knee-replacement surgery patients, the authors conducted a large randomized study on the integrated use of erythropoietin, cell
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| H5166-10UG | 04061833797778 |
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Hi, we recently ordered this product, and I wonder how much international unit for this product of 10ug?
1 answer-
Helpful?
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