The β-Glucosidase from almonds should be stored in a solvent at -80°C for up to 6 months, or at -20°C for up to 1 month.
G4511
β-Glucosidase from almonds
lyophilized powder, 10-50 units/mg solid
Synonyma:
β-D-Glucoside glucohydrolase
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
100 units | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 4 980,00 Kč |
250 units | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 9 800,00 Kč |
1000 units | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 32 500,00 Kč 27 625,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-589-7
MDL number:
Specific activity:
10-50 units/mg solid
4 980,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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lyophilized powder
Quality Segment
specific activity
10-50 units/mg solid
mol wt
50-75 kDa
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
impurities
salt, essentially free
greener alternative category
, Enabling
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.
β-Glucosidase from almonds belongs to the family 1 of the glycoside hydrolases. Almonds contain prunasin hydrolases (PH) and amygdalin hydrolase. Nine types of PHs are associated with almonds. PHs have signal peptide sequence and correspond to molecular weight in the range 50 to 75 kDa.[1] PHs also have ITENG, NEP and INKKGIEYY motifs conserved and have N-glycosylation sites.[1]
Application
β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.
β-Glucosidase from almonds has been used:
- in enzyme inhibition studies by 1,5-dideoxy-1,5-imino-d-xylitol(DIX) derivates
- as a control in for comparison of soybean isofavone glycosides degradation by β-Glucosidase from Talaromyce leycettanus
- as a medium component during saccharification and fermentation of yeast
Biochem/physiol Actions
βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.
Prunasin hydrolase (PH) is a β-glucosidase that degrades prunasin to mandelonitrile and glucose. Amygdalin hydrolase hydrolyzes amygdalin to prunasin and glucose.[1] β-Glucosidase is mesostable and is sensitive to temperature at 60 °C and above. It is highly sensitive to high pressure.
Preparation Note
Chromatographically purified
Other Notes
One unit will liberate 1.0 μmole of glucose from salicin per min at pH 5.0 at 37 °C.
1 of 1
Tato položka | |||
|---|---|---|---|
| specific activity 10-50 units/mg solid | specific activity ≥4 U/mg | specific activity - | specific activity ≥2 units/mg solid |
| form lyophilized powder | form powder | form buffered aqueous glycerol solution | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 50-75 kDa | mol wt Mr ~135000 | mol wt - | mol wt 135 kDa |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 300 |
| impurities salt, essentially free | impurities - | impurities - | impurities salt, essentially free |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Skladovací třída
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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