D9909

Dextran Sucrase from Leuconostoc mesenteroides

lyophilized powder, ≥100 units/mg protein

• Číslo CAS: 9032-14-8
• Číslo EC: 2.4.1.5 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00130936
• Specific activity: ≥100 units/mg protein
• Biological source: bacterial (Leuconostoc mesenteroides)

Vlastnosti

• biological source: bacterial (Leuconostoc mesenteroides)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥100 units/mg protein
• composition: Protein, ~15% Lowry
• solubility: H₂O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
• storage temp.: −20°C

Popis

General description

Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.

Application

Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.

Dextran Sucrase from Leuconostoc mesenteroides has been used:

• in immobilization on shirasu porous membrane (SPG) for dextran production
• in the enzymatic synthesis of dextran nanoparticles at various pH range
• in the immobilization with hydroxyapatite for dextran production

The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.

Biochem/physiol Actions

Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages.[1] The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad.[2] The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.[3]

Physical form

Lyophilized powder containing dextran, MES buffer salts and CaCl₂

Analysis Note

Chromatographically purified

Other Notes

One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)