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D8537

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonyma:

DPBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

product name

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

  • for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.
  • to wash the wells of a microtiter plate.
  • to wash the cells before resuspending them in lysis buffer for DNA isolation.
  • as a component of embryo holding medium for vitrification of embryos.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

also commonly purchased with this product

Č. produktu
Popis
Stanovení ceny

D1145

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

R8758

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

F1051

Fetal Bovine Serum, Canada origin, sterile-filtered, suitable for cell culture

CLS430641

Corning® cell culture flasks, surface area 75 cm2, canted neck, cap (vented)

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Protokoly

Subculture of Adherent Cell Lines

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Rat Skeletal Muscle Cells (RSkMC) Culture Protocol

Technical information for working with rat skeletal muscle cells including thawing, subculturing and cryopreservation

Human Follicle Dermal Papilla Cells (HFDPC) Culture Protocol

Technical information for working with human follicle dermal papilla cells including thawing, subculturing and cryopreservation.

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