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D8537

Dulbecco’s Phosphate Buffered Saline

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Dulbecco’s Phosphate Buffered Saline
1 of 6 reviewers received a sample product or took part in a promotion

Synonyma:

DPBS

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Velikost baleníSkladová položkaDostupnostCena
100 mL
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444,00 Kč
500 mL
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809,00 Kč
1 L
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1 740,00 Kč
6 x 500 mL
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4 780,00 Kč
6 x 1 L
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8 740,00 Kč
24 x 500 mL
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16 800,00 Kč

O této položce

UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75
MDL number:

444,00 Kč


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Název produktu

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

storage temp.

room temp

General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

  • for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.
  • to wash the wells of a microtiter plate.[1]
  • to wash the cells before resuspending them in lysis buffer for DNA isolation.[2]
  • as a component of embryo holding medium for vitrification of embryos.

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Tato položka
59321C59331C59300C
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

300

Quality Level

400

Quality Level

400

Quality Level

500

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-


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Skladovací třída

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

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Zobrazit všechny protokoly

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Použití lipidy modifikovaných olig MULTI-seq, protokol a průvodce řešením problémů pro PCR testy a sekvenační aplikace.

Související obsah

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Globální číslo obchodní položky

Skladová položkaGTIN
D8537-6X500ML04061838259400
D8537-100ML04061833589724
D8537-6X1L04061838259394
D8537-500ML04061838259387
D8537-1L04061838259370
D8537-24X500ML04061833589731

Questions

1–10 of 11 Questions  
  1. Eu gostaria de saber a osmolaridade da solução de DMPBS.

    1 answer
    1. This product has an osmolarity range of 275–304 mOsm/kg.

      Helpful?

  2. Quería saber si éste producto se puede considerar RNAsa free para realizar durante la hibridación in situ

    1 answer
    1. This product is a sterile-filtered M Quality Level 300 product. It is not molecular biology grade and does not have specifications such as RNase and DNase free. Therefore, this product cannot be considered RNase free for use in in situ hybridization.

      Helpful?

  3. Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide

    1 answer
    1. This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.

      Helpful?

  4. Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?

    1 answer
    1. This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.

      Helpful?

  5. What is the concentration of the product in Molarity?

    1 answer
    1. This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
      Product D8537 contains:
      Potassium chloride: 0.2 g/l
      Potassium phosphate monobasic: 0.2 g/l
      Sodium chloride: 8 g/l
      Sodium Phosphate dibasic: 1.15 g/l

      Please see the link below for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdf

      Helpful?

  6. What is the bottle thread type on the 1L bottle (D8537-1L)

    1 answer
    1. The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
      Thread Type 38-430
      Thread Diameter 37.3 mm

      Helpful?

  7. What is the formulation for Product D8537, Dulbecco's Phosphate?

    1 answer
    1. The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.

      Helpful?

  8. Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

    1 answer
    1. This is a 1X concentration. The 10X concentration is listed under product D1408.

      Helpful?

  9. What is the pH of a solution of Product D8537, Dulbecco's Phosphate?

    1 answer
    1. The pH will be in the range of 7.1-7.5, according to the specification sheet.

      Helpful?

  10. What is a Salt Toxicity Test?

    1 answer
    1. The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted.  They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour.  The cells are counted and %viability determined.  The cells are then cultured for an additional 7 hours (total of 8 hours).  The cells are counted again and the % viability determined.  The viability at 1 hr has to be >95% of the original viability.  The viability at 8 hrs must be >75% of the original viability.

      Helpful?

1–10 of 11 Questions  

Reviews

Active Filters

  1. Atlanta, GA
    • Reviews 3
    • Votes 95
    5 out of 5 stars.

    All-purpose DPBS

    It's DPBS with calcium-magnesium modification. Worked pretty well for me for various applications.

    Helpful?

  2. 5 Ratings-only reviews