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BCA1

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

Synonyma:

BCA protein assay

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Velikost baleníSkladová položkaDostupnostCena
1 kit
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
12 400,00 Kč

O této položce

NACRES:
NA.32
UNSPSC Code:
12352106

12 400,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
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Technický servis
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Quality Level

200

usage

sufficient for ~500 assays

storage temp.

2-8°C

Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Biochem/physiol Actions

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Features and Benefits

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Tato položka
QPBCAB964357697
Bicinchoninic Acid Kit for Protein Determination for 200-1000 μg/ml protein

Sigma-Aldrich

BCA1

Bicinchoninic Acid Kit for Protein Determination

QuantiPro™ BCA Assay Kit for 0.5-30 μg/ml protein

Sigma-Aldrich

QPBCA

QuantiPro™ BCA Assay Kit

Bicinchoninic Acid solution

Supelco

B9643

Bicinchoninic Acid solution

Sigma-Aldrich

Sigma-Aldrich

57697

Advanced Protein Assay

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

usage

sufficient for ~500 assays

usage

-

usage

-

usage

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Pouze součásti soupravy

Č. produktu
Popis
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

Skladovací třída

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
0000552224
0000539848
0000552224
0000539848
0000502790

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Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

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Sortimentní položky

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

Související obsah

Nástroje pro kvantifikaci proteinů

K dispozici jsou produkty pro tradiční i alternativní techniky kvantifikace proteinů, včetně BCA, Bradfordovy, Lowryho a dalších.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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Globální číslo obchodní položky

Skladová položkaGTIN
BCA1-1KT04061835571055

Questions

1–4 of 4 Questions  

  1. Good morning. Is the "Bicinchoninic Acid Kit for Protein Determination" complete? i.e. does it contain all the necessary reactants? Is there a need to buy plates, wells or other? Thank you.

    1 answer

    1. This kit does require the use of materials that are dependant on the assay format used, but not provided in the kit. These items are as follows:

      • Spectrophotometer capable of accurately measuring absorbance in the 560 nm region
      • 96-well plates, such as Cat. No. CLS9017
      • 96-well plate sealing film: Cat. No. Z369667

      OR

      • Test tubes, 13 × 100 mm: Cat. No. CLS980013
      • 1 mL Disposable Plastic Cuvettes: Cat. No. C5416

      Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

      Helpful?

  2. Can the kit be used for measuring the protein content of marine bacteria grown in artificial sea water?

    1 answer

    1. This product has not been tested for use with marine bacteria in artificial sea water. However, the publication below used the Bicinchoninic acid method to measure total protein in planktonic marine bacteria, indicating that this kit may be suitable.
      Determination of Total Protein Content of Bacterial Cells by SYPRO Staining and Flow Cytometry
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC91485/

      Note that there are a number of potential interfering substances that may impact results. Please see the link below to review the product datasheet, including a list of interfering substances beginning on page 4.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

      Helpful?

  3. Does this kit rely on naked-eye colorimetric detection or does it require a fluorescent microscope?

    1 answer

    1. The kit is based on colorimetric, not fluorometric, detection. The color intensity should be measured at A562nm

      Helpful?

  4. Can a standard OD plate reader be used for the BCA1-1KT Kit, which offers a choice of assay tubes and 96-well plates as mentioned in the technical description? Additionally, would the Test tubes, 13 × 100 mm, Catalog Number CLS980013 not be needed?

    1 answer

    1. If a standard ELISA plate reader capable of measuring absorbance in the 560nm region is being used, the 96-well format should be suitable. The test tubes are recommended for larger volume analysis with cuvettes, but if the 96-well format is used, they will not be needed for the samples. However, they are also being utilized to make the BSA protein standard curve dilutions. If there is an alternative method for making the serial standard protein dilutions that ensures thorough mixing and utilizes a low-binding surface to prevent protein absorption, such as borosilicate glass and/or polypropylene tubes, that will be suitable as well.

      Helpful?

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