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B2025

Sigma-Aldrich

Brilliant Blue G - Colloidal Concentrate

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

form

solution

storage temp.

room temp

General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
  • protein fragments post in vitro pepsin digestion
  • ovarian tumor fluid proteome prior to profiling
  • the transcription factor (TFIID) complex from Saccharomyces cerevisiae

Reconstitution

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

related product

Č. produktu
Popis
Stanovení ceny

G1041

EZBlue™ Gel Staining Reagent

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Navštívit knihovnu dokumentů

Julie Ducreux et al.
Bioconjugate chemistry, 19(10), 2088-2094 (2008-09-24)
PEGylation of antibodies is known to increase their half-life in systemic circulation, but nothing is known regarding whether PEGylation can improve the inhibitory potency of antibodies against target receptors. In this paper, we have examined this question using antibodies directed
A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
Thomas K, et al.
Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
Dyballa N and Metzger S
Journal of Visualized Experiments (2009)
Roy Auty et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
Antonis Ververis et al.
Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here
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Protokoly

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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