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A8706

Avidin from egg white

recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Synonyma:

AVID_CHICK

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

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Velikost baleníSkladová položkaDostupnostCena
5 mg
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
5 300,00 Kč

O této položce

Číslo CAS:
1405-69-2
UNSPSC Code:
12352202
EC Number:
215-783-6
NACRES:
NA.56
MDL number:
MFCD00130572
Form:
powder
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in corn
Mol wt:
glycoprotein 66 kDa, subunit 16 kDa

5 300,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
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recombinant

expressed in corn

Quality Level

200

assay

≥80% protein basis (biuret)

form

powder

specific activity

≥12 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa, subunit 16 kDa

technique(s)

ELISA: suitable, immunoblotting: suitable, immunoelectrophoresis: suitable

UniProt accession no.

P02701

storage temp.

2-8°C

Gene Information

chicken ... AVD(396260)

General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.[1]

Preparation Note

Affinity purified

Other Notes

One unit will bind 1.0 μg of d-biotin.

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Tato položka
A7294A3151A5378
Avidin from egg white recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Sigma-Aldrich

A8706

Avidin from egg white

Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Albumin from chicken egg white lyophilized powder, ≥90% (agarose gel electrophoresis)

Sigma-Aldrich

A5378

Albumin from chicken egg white

technique(s)

ELISA: suitable, immunoelectrophoresis: suitable, immunoblotting: suitable

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

technique(s)

direct ELISA: 1:50,000

technique(s)

bioconjugation: suitable

recombinant

expressed in corn

recombinant

-

recombinant

-

recombinant

-

assay

≥80% protein basis (biuret)

assay

-

assay

-

assay

≥90% (agarose gel electrophoresis)

mol wt

glycoprotein 66 kDa, subunit 16 kDa

mol wt

-

mol wt

-

mol wt

44287 Da by calculation

form

powder

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized powder

Gene Information

chicken ... AVD(396260)

Gene Information

-

Gene Information

-

Gene Information

chicken ... OVAL(396058)

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Skladovací třída

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Osvědčení o analýze (COA)

Lot/Batch Number
0000496586
0000496586
0000434530
0000434530
0000316992

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Sofia H L Frost et al.
Cancer biotherapy & radiopharmaceuticals, 28(2), 108-114 (2012-12-13)
Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinylated, succinylated poly-l-lysine ((211)At-B-PLsuc) was compared with conventional radioimmunotherapy (RIT) using (211)At-labeled MX35 in a nude mouse model. Mice were inoculated i.p.
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Journal of plant physiology, 258-259, 153359-153359 (2021-01-19)
Recombinant proteins play an important role in many areas of our lives. For example, recombinant enzymes are used in the food and chemical industries and as high-quality proteins for research, diagnostic and therapeutic applications. The production of recombinant proteins is
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Globální číslo obchodní položky

Skladová položkaGTIN
A8706-5MG04061833400562

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