Hlavní dokumenty

Zobrazit celou dokumentaci

Přeskočit na

A2356

Phosphatase, Alkaline from bovine intestinal mucosa

Name: Phosphatase, Alkaline from bovine intestinal mucosa
Brand: SIGMA
Price: 7680 CZK

≥5,500 DEA units/mg protein

1 of 1 reviewers received a sample product or took part in a promotion

Synonyma:

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

Změnit zobrazení

Velikost balení	Skladová položka	Dostupnost	Cena
10000 units		Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	7 680,00 Kč
50000 units		Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	31 000,00 Kč

O této položce

Číslo CAS:

9001-78-9

UNSPSC Code:

12352204

eCl@ss:

42010105

NACRES:

NA.54

Číslo EC:

3.1.3.1 (BRENDA, IUBMB)

MDL number:

MFCD00131849

Specific activity:

≥5,500 DEA units/mg protein

Concentration:

≥10 mg/mL

7 680,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

Technický servis

Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.

Dovolte nám, abychom vám pomohli

Vlastnosti

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

Quality Level

200

specific activity

≥5,500 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

≥10 mg/mL

storage temp.

2-8°C

Popis

General description

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Porovnat podobné položky

Zobrazit úplné porovnání

Zobrazit rozdíly

1 of 1

Tato položka	P0114	P7923	P7640
Sigma-Aldrich A2356 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P0114 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P7923 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P7640 Phosphatase, Alkaline from bovine intestinal mucosa
specific activity ≥5,500 DEA units/mg protein	specific activity ≥5,700 DEA units/mg protein	specific activity ≥4,000 DEA units/mg protein	specific activity ≥10 DEA units/mg solid
form (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)	form (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)	form buffered aqueous glycerol solution	form lyophilized powder
concentration ≥10 mg/mL	concentration ≥10 mg/mL	concentration ≥10.0 mg/mL	concentration -
mol wt dimer ~160 kDa	mol wt dimer ~160 kDa	mol wt dimer ~160 kDa	mol wt dimer ~160 kDa
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Still not finding the right product?

Explore all of our products under Phosphatase, Alkaline from bovine intestinal mucosa

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Skladovací třída

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

Nevidíte správnou verzi?

Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

Vyhledat COA

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Protokoly

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Související obsah

Product information sheet

Monocyte-Specific Knockout of C/ebpα Results in Osteopetrosis Phenotype, Blocks Bone Loss in Ovariectomized Mice, and Reveals an Important Function of C/ebpα in Osteoclast Differentiation and Function.

Wei Chen et al.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(4), 691-703 (2017-11-18)

CCAAT/enhancer-binding protein α (C/ebpα) is critical for osteoclastogenesis by regulating osteoclast (OC) lineage commitment and is also important for OC differentiation and function in vitro. However, the role of C/ebpα in postnatal skeletal development has not been reported owing to

Intestinal Alkaline Phosphatase Exerts Anti-Inflammatory Effects Against Lipopolysaccharide by Inducing Autophagy.

Sudha B Singh et al.

Scientific reports, 10(1), 3107-3107 (2020-02-23)

Intestinal alkaline phosphatase (IAP) regulates bicarbonate secretion, detoxifies lipopolysaccharide (LPS), regulates gut microbes, and dephosphorylates proinflammatory nucleotides. IAP also exhibits anti-inflammatory effects in a Toll-like Receptor-4 (TLR-4) dependent manner. However, it is not known whether IAP induces autophagy. We tested

Core-binding factor beta is required for osteoblast differentiation during fibula fracture healing.

Tuanmao Guo et al.

Journal of orthopaedic surgery and research, 16(1), 313-313 (2021-05-16)

Growing evidence has implicated core-binding factor beta (Cbfb) as a contributor to osteoblast differentiation, which plays a key role in fracture healing. Herein, we aimed to assess whether Cbfb affects osteoblast differentiation after fibula fracture. Initially, we established a Cbfb

Zobrazit všechny související dokumenty

Globální číslo obchodní položky

Skladová položka	GTIN
A2356-10KU	04061833350010
A2356-50KU	04061832754512

Questions

: 1 Review This action will navigate to reviews.

1 of 1 reviewers received a sample product or took part in a promotion

Reviews

Rating Snapshot

Select a row below to filter reviews.

Average Customer Ratings

Overall, average rating value is 2 of 5.

Active Filters

1 Ratings-only review

Hlavní dokumenty

Přeskočit na

Phosphatase, Alkaline from bovine intestinal mucosa

≥5,500 DEA units/mg protein

Vybrat velikost

O této položce

A2356-10KU

7 680,00 Kč

form

Quality Level

specific activity

mol wt

concentration

storage temp.

General description

Application

Biochem/physiol Actions

Physical form

Analysis Note

Other Notes

Porovnat podobné položky

Tato položka

Still not finding the right product?

Bezpečnostní informace

pictograms

signalword

hcodes

pcodes

Hazard Classifications

Skladovací třída

wgk

flash_point_f

flash_point_c

ppe

Dokumentace

Osvědčení o analýze (COA)

Již tento produkt vlastníte?

Protokoly a sortimentní položky

Protokoly

Související obsah

Zobrazit náhled zkontrolovaných dokumentů

Globální číslo obchodní položky

Questions

Reviews

Rating Snapshot

Average Customer Ratings

Active Filters

1 Ratings-only review