94403

Hoechst 33258 solution

1 mg/mL in H₂O, ≥98.0% (HPLC)

• Synonyma: bisBenzimide H 33258 solution
• Empirický vzorec (Hillův zápis): C₂₅H₂₄N₆O · 3HCl
• Číslo CAS: 23491-45-4
• Molekulová hmotnost: 533.88
• MDL number: MFCD29074011
• UNSPSC Code: 12171500
• PubChem Substance ID: 329770334
• NACRES: NA.32
• Assay: ≥98.0% (HPLC)
• Form: liquid

Vlastnosti

• Quality Segment: 100
• assay: ≥98.0% (HPLC)
• form: liquid
• mol wt: 533.88 g/mol
• concentration: 1 mg/mL in H₂O
• color: colorless, very light green-yellow to dark green-yellow, very light yellow to dark yellow
• pH: 6.0-8.0 (25 °C)
• solubility: DMF: soluble, H₂O: soluble
• density: 1.00 g/cm³
• fluorescence: λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
• storage temp.: 2-8°C
• SMILES string: Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
• InChI key: SMNPLAKEGAEPJD-UHFFFAOYSA-N

Popis

General description

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. [1]It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.[2]

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA.[3] Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.[4]

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable