A specific method for analyzing this standard is not available. However, the following protocol can be used to analyze the amino acid standard solution using LC-MS/MS:
https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/analysis-of-20-underivatized-amino-acids
. A representative chromatogram of the elution of the amino acids is also provided in the protocol.
A9781
Amino acid standards for protein hydrolysates
analytical standard
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Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 5 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 4 220,00 Kč |
O této položce
UNSPSC Code:
85151701
NACRES:
NA.24
eCl@ss:
32160406
4 220,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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analytical standard
composition
L-Alanine , Ammonium chloride , L-Arginine , L-Aspartic acid , L-Cystine , L-Glutamic acid , Glycine , L-Histidine , L-Isoleucine , L-Leucine , L-Lysine , L-Methionine , L-Phenylalanine , L-Proline , L-Serine , L-Threonine , L-Tryosine , L-Tryptophan , L-Valine
analyte chemical class(es)
amino acids, peptides, proteins
technique(s)
HPLC: suitable, gas chromatography (GC): suitable
color
colorless
application(s)
food and beverages
format
neat
storage temp.
2-8°C
Application
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Other Notes
Amino acids listed are 0.5 μmole/mL except L-cystine at 0.25 μmole/mL in 0.2 N sodium citrate, pH 2.2.
1 of 1
Tato položka | |||
|---|---|---|---|
| format neat | format neat | format multi-component solution | format neat |
| technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| grade analytical standard | grade analytical standard | grade analytical standard | grade analytical standard |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| analyte chemical class(es) amino acids, peptides, proteins | analyte chemical class(es) amino acids, peptides, proteins | analyte chemical class(es) amino acids, peptides, proteins | analyte chemical class(es) amino acids, peptides, proteins |
| color colorless | color - | color - | color colorless |
Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How to analyze the chromatogram and quantify the amino acids in our sample as well as in the standard ? Is there any chromatogram for this standard solution ?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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I am looking for the amino acid's solution for HPLC in diagnostic lab as a QC. The mixture approximately contains approx. 40amino acid and concentration is 2.5mM or higher than that
1 answer-
This product contains 19 amino acids, each at a concentration of 0.5 mM, except for L-cystine, which is at 0.25 mM. This is less than the required concentration and with fewer analytes. Contact the technical service group in the region for assistance in finding a suitable standard for the application.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Good afternoon. I am searching for a mixture of aminoacids to built method in HPLC to identify several amino acids. I need to know their concentration in the mixture to construct a calibration curve. Will this the best option? thanks
1 answer-
Amino Acids Mix Solution - 79248-5X2ML is suitable for this application. The COA can be found here, https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/PDF746486.pdf.
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A9781 is it suitable for PDA detector HPLC
1 answer-
Product A9781-5ML is suitable for HPLC analysis. While the analysis of amino acids using a PDA/UV requires a lot of steps, it is still possible and practical. However, most HPLC methods for amino acid quantification uses Mass Spectrometry (MS) or Charged Aerosol Detector (CAD) detectors.
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