NADP-RO

NADP

Disodium salt

• Synonyma: β-Nicotinamide adenine dinucleotide phosphate disodium salt, β-NADH phosphate disodium salt, β-NADP-Na₂, Triphosphopyridine nucleotide disodium salt
• Empirický vzorec (Hillův zápis): C₂₁H₂₆N₇Na₂O₁₇P₃
• Číslo CAS: 24292-60-2
• Molekulová hmotnost: 787.37
• UNSPSC Code: 12352302
• PubChem Substance ID: 329818702
• MDL number: MFCD00065390
• NACRES: NA.54
• Assay: ≥97%
• Form: powder
• Storage temp.: 2-8°C

Vlastnosti

• assay: ≥97%
• form: powder
• mol wt: M_r 743.4, M_r 787.4
• packaging: pkg of 1 g (10128058001), pkg of 100 mg (10128031001), pkg of 5 g (10240354001), pkg of 500 mg (10128040001)
• manufacturer/tradename: Roche
• pH: 4.0 ± 1.0(10mg/mL)
• λmax: 260 nm
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: [Na+].[Na+].NC(=O)c1ccc[n+](c1)C2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](OP(O)([O-])=O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
• InChI: 1S/C21H28N7O17P3.2Na/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32;;/h1-4,7-8,10-11,13-16,20-21,29-31H,5-6H2,(H7-,22,23,24,25,32,33,34,35,36,37,38,39);;/q;2*+1/p-2/t10-,11-,13-,14-,15-,16-,20?,21-;;/m1../s1
• InChI key: WSDDJLMGYRLUKR-FHQWUTKFSA-L

Popis

General description

Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair.[1] The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.[2]

Application

The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen.[3] It has also been used for the hexokinase assay.[4]

Preparation Note

Storage conditions (working solution): 2 to 8 °C
10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C

Analysis Note

Absorbance coefficients: 18.0 x 10³ [l x mol^-1 x cm^-1] at 260 nm, 5.9 x 10³ [l x mol^-1 x cm^-1] at 333 nm (CN complex)

Absorbance at 340 nm: Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A₃₄₀ approximately 0.100.

Absorbance at 360 nm: Aqueous solution of NADP, 10 mg/ml: A₃₆₀ approximately 0.200-0.250.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Bezpečnostní informace

• Skladovací třída: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable