These cell lines were selected using hygromycin resistance.
SCC007
ReNcell CX Human Neural Progenitor Cell Line
ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.
Synonyma:
Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells
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Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|
O této položce
UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203
Biological source:
human
Informace o stanovených cenách a dostupnosti nejsou v současnosti dostupné.
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohlibiological source
human
Quality Segment
manufacturer/tradename
Chemicon®, ReNcell
technique(s)
cell culture | stem cell: suitable
input
sample type neural stem cell(s)
shipped in
liquid nitrogen
storage temp.
-140 to -196°C
General description
Product Source: Human cortical brain tissue
ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.
ReNcell Now Used in a 3D Cell Model of Alzheimer′s Disease!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease. Nature. 2014 Nov 13;515(7526):274-8.
(Published in Nature)
ReNcell Now Used in a 3D Cell Model of Alzheimer′s Disease!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease. Nature. 2014 Nov 13;515(7526):274-8.
(Published in Nature)
Application
Research Category
Stem Cell Research
Stem Cell Research
Biochem/physiol Actions
Neural Stem Cells
Preparation Note
When stored at the recommended storage conditions (liquid nitrogen), ReNcell CX cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
Analysis Note
Each lot of ReNcell CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.
Other Notes
> 1x106 viable ReNcell CX cells
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
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Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Protokoly
Kultivační protokoly krok za krokem pro kultivaci nervových kmenových buněk včetně izolace, expanze, diferenciace a charakterizace NSC.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Sortimentní položky
Odvození a charakterizace funkčních lidských oligodendrocytárních progenitorových buněk (OPC) odvozených z neurálních kmenových buněk, které účinně myelinizují primární neurony v kultuře.
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.
Související obsah
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
ReNcell® VM and ReNcell® CX are two well-established neural stem cell lines derived from developing human brains. ReNcell® VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. This article describes the characteristics and differentiation of ReNcell® lines. Results of calcium and membrane potential changes in response to various ligands are also shown. The convenience to maintain in culture and flexibility to differentiate to individual scientists’ needs make ReNcell® lines the ideal platform for research and discovery.
Mia C Borlongan et al.
Neuromolecular medicine, 23(4), 540-548 (2021-04-09)
The present in vitro study showed that IL-2/IL-2R antibody complex facilitates Treg-induced neuroprotection in the oxygen glucose deprivation/reoxygenation (OGD/R) model of stroke. First, we examined the role of IL-2/IL-2R-treated Tregs in OGD/R-exposed rat primary cortical cells (PCCs), which represent the
Greg Maguire et al.
Physiological reports, 7(9), e14072-e14072 (2019-05-03)
Evidence suggests that adult stem cell types and progenitor cells act collectively in a given tissue to maintain and heal organs, such as muscle, through a release of a multitude of molecules packaged into exosomes from the different cell types.
Liang Luo et al.
Cells, 10(3) (2021-03-07)
Human adipose-derived stromal cells (ADSCs) are receiving unprecedented attention as a potential cellular source for regenerative medicine-based therapies against various diseases and conditions. However, there still have significant issues concerning the translational development of ADSC-based therapies, such as its heterogeneity
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| SCC007 | 04053252358609 |
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Hello, are these cell lines Puromycin or Hygromycin resistant?
1 answer-
Helpful?
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