MABE282
Anti-c-Myc Antibody, clone 9E10
clone 9E10, from mouse
Synonyma:
Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, HLHe39, Proto-oncogene c-Myc, Transcription factor p64
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Doporučené produkty
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9E10, monoclonal
species reactivity
human
technique(s)
ChIP: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYC(4609)
Související kategorie
General description
c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell differentiation and cell proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling to increase accessibility of transcriptional complexes to target DNA sequences. The expression of c-Myc is controlled by growth factors. Several studies have reported abnormal expression of c-Myc in various cancers including Burkitt’s lymphoma.
Immunogen
Linear peptide corresponding to human c-Myc.
Application
Anti-c-Myc Antibody, clone 9E10 is a Mouse Monoclonal Antibody for detection of c-Myc also known as Myc proto-oncogene protein, HLHe39, Transcription factor p64 & has been validated in WB, ChIP.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot Analysis: A representative lot from an independent laboratory detected c-Myc in various cell lysates (Evan, G. I., et al. (1985). Mol Cell Biol. 5(12):3610-3616).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected c-Myc in ChIP (Sanyal, K., et al. (2004). Proc Natl Acad Sci USA. 101(31):11374-11379).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected c-Myc in ChIP (Sanyal, K., et al. (2004). Proc Natl Acad Sci USA. 101(31):11374-11379).
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected c-Myc in 10 µg of HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected c-Myc in 10 µg of HeLa cell lysate.
Target description
~51 kDa observed. Uniprot describes two isoforms at ~49 kDa and ~51 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ještě jste nenalezli správný produkt?
Vyzkoušejte náš produkt Nástroj pro výběr produktů.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Zákazníci si také prohlíželi
Samuel Rommelaere et al.
Cell reports, 27(3), 886-899 (2019-04-18)
In ectotherms, increased ambient temperature requires the organism to consume substantial amounts of energy to sustain a higher metabolic rate, prevent cellular damage, and respond to heat stress. Here, we identify a heat-inducible apolipoprotein required for thermal acclimation in Drosophila.
Yang Sun et al.
Nature communications, 13(1), 6744-6744 (2022-11-09)
Targeting TEAD autopalmitoylation has been proposed as a therapeutic approach for YAP-dependent cancers. Here we show that TEAD palmitoylation inhibitor MGH-CP1 and analogues block cancer cell "stemness", organ overgrowth and tumor initiation in vitro and in vivo. MGH-CP1 sensitivity correlates
Justina Rutkauskaite et al.
iScience, 25(7), 104515-104515 (2022-06-24)
High-throughput screening and enrichment of antibody-producing cells have many important applications. Herein, we present a droplet microfluidic approach for high-throughput screening and sorting of antibody-secreting cells using a Förster resonance electron transfer (FRET)-based assay. The FRET signal is mediated by
Thibault Houles et al.
Cell death discovery, 9(1), 459-459 (2023-12-17)
CDK12 is a transcriptional cyclin-dependent kinase (CDK) that interacts with cyclin K to regulate different aspects of gene expression. The CDK12-cyclin K complex phosphorylates several substrates, including RNA polymerase II (Pol II), and thereby regulates transcription elongation, RNA splicing, as
David Z Kochan et al.
Journal of cell science, 134(15) (2021-08-06)
Gene expression involves regulation of chromatin structure and transcription, as well as processing of the transcribed mRNA. While there are feedback mechanisms, it is not clear whether these include crosstalk between chromatin architecture and mRNA decay. To address this, we
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis.