LSKMAGG
PureProteome Protein G Magnetic Bead System
The PureProteome Protein G Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability
Synonyma:
Protein G Magnetic Beads, PureProteome Magnetic Beads
Vybrat velikost
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 2 x 1 mL | Odhaduje se, že bude odesláno dnesodAreál Kühne+Nagel spol. s r.o. | 8 520,00 Kč | |
| 10 mL | Odhaduje se, že bude odesláno dnesodAreál Kühne+Nagel spol. s r.o. | 40 500,00 Kč |
O této položce
packaging
pkg of 2 × 1 mL
manufacturer/tradename
PureProteome
technique(s)
depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable
particle size
10 μm
capacity
2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)
shipped in
wet ice
General description
Application
- serum depletion
- immunoprecipitation
- protein purification
- for isolation of the immunoglobulin G (IgG) fraction from a small volume of plasma
- for co-immunoprecipitation (Co-IP)
Features and Benefits
- PureProteome™ Magnetic Beads are very well suited for fully automated purification process using the KingFisher Duo particle processor, it is not dependent on the cell lysate clarification step
- The entire process is reproducible, and the results are comparable to that of a standard protocol using the PureProteome Magnetic Stand
- PureProteome Magnetic Beads is compatible with any automated system for low, medium, or high-throughput sample preparation.
Legal Information
1 of 1
Tato položka | |||
|---|---|---|---|
| manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome |
| particle size 10 μm | particle size 10 μm | particle size 10 μm | particle size 10 μm |
| packaging pkg of 2 × 1 mL | packaging pkg of 2 × 1 mL | packaging pkg of 10 mL | packaging pkg of 10 mL |
| capacity 2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG) | capacity 1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG) | capacity 1-5.5 mg/mL, bead suspension binding capacity (protein) | capacity - |
| technique(s) depletion: suitable (serum), protein purification: suitable, immunoprecipitation (IP): suitable | technique(s) depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable | technique(s) protein purification: suitable (histidine tagged recombinant protein) | technique(s) depletion: suitable (serum), protein purification: suitable |
| shipped in wet ice | shipped in wet ice | shipped in ambient | shipped in wet ice |
Skladovací třída
12 - Non Combustible Liquids
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
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Sortimentní položky
PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.
Související obsah
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| LSKMAGG10 | 04053252323188 |
| LSKMAGG02 | 04053252373053 |