This product is the inactive form of the kinase, obtained after purification by Ni-NTA agarose from insect cells. In this initial form after purification, the kinase exhibits negligible activity. No inhibitors or mutations are used to generate the inactive form.
14-279
Akt1/PKBα Protein, inactive, 50 g
Unactive, N-terminal His6-tagged recombinant full-length human Akt1, for use in Kinase Assays.
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Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 50 μg | Zkontrolujte dostupnost v košíku | 14 400,00 Kč |
O této položce
UNSPSC Code:
12352202
NACRES:
NA.26
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Mol wt:
Mw 59 kDa
Technický servis
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human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 59 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
N-terminal His6-tagged recombinant full-length human Akt1
Analysis Note
routinely evaluated by phosphorylation of Crosstide
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Tato položka | |||
|---|---|---|---|
| technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (IP-kinase), activity assay: suitable (kinase) |
| recombinant expressed in baculovirus infected Sf21 cells | recombinant expressed in Sf21 cells | recombinant expressed in baculovirus infected Sf21 cells | recombinant expressed in baculovirus infected Sf21 cells |
| biological source human | biological source human | biological source human | biological source human |
| mol wt Mw 59 kDa | mol wt Mw 49.2 kDa | mol wt Mw 45 kDa | mol wt Mw 59.9 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Skladovací třída
10 - Combustible liquids
wgk
WGK 2
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
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Chia-Chen Chen et al.
Developmental cell, 18(4), 592-604 (2010-04-24)
FoxO transcription factors and TORC1 are conserved downstream effectors of Akt. Here, we unraveled regulatory circuits underlying the interplay between Akt, FoxO, and mTOR. Activated FoxO1 inhibits mTORC1 by TSC2-dependent and TSC2-independent mechanisms. First, FoxO1 induces Sestrin3 (Sesn3) gene expression.
Dos D Sarbassov et al.
Molecular cell, 22(2), 159-168 (2006-04-11)
The drug rapamycin has important uses in oncology, cardiology, and transplantation medicine, but its clinically relevant molecular effects are not understood. When bound to FKBP12, rapamycin interacts with and inhibits the kinase activity of a multiprotein complex composed of mTOR
Chang Hwa Jung et al.
Autophagy, 7(10), 1212-1221 (2011-07-29)
ULK1 (Unc51-like kinase, hATG1) is a Ser/Thr kinase that plays a key role in inducing autophagy in response to starvation. ULK1 is phosphorylated and negatively regulated by the mammalian target of rapamycin complex 1 (mTORC1). Previous studies have shown that
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| 14-279 | 04053252624872 |
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Is this prduct a kinase-dead Akt1 mutant?
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