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1.10426

Chromocult^® Coliform Agar

Name: Chromocult<SUP>®</SUP> Coliform Agar
Brand: MM
Price: 16100 CZK

ChromoCult^®, according to ISO 9308, EPA, AOAC^®, granular, for coliforms, for Escherichia coli, pack of 500 g

Synonyma:

Coliform Agar, Chromogenic E. coli Agar, Chromogenic Coliform Agar

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Velikost balení	Skladová položka	Dostupnost	Cena
500 g		Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	16 100,00 Kč

O této položce

Číslo CAS:

1473387-52-8

MDL number:

MFCD00008872

NACRES:

NA.74

UNSPSC Code:

41106213

Manufacturer/tradename:

ChromoCult^®

Agency:

AOAC^®, EPA, according to ISO 9308

Ph:

6.6-7.0 (25 °C, 26.5 g/L in H₂O)

Form:

granular

Suitability:

Escherichia coli, coliforms

Application(s):

food and beverages
microbiology

16 100,00 Kč

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Vlastnosti

Quality Level

200

agency

AOAC^®, EPA, according to ISO 9308

sterility

non-sterile

form

granular

packaging

pack of 500 g

manufacturer/tradename

ChromoCult^®

technique(s)

microbiological culture: suitable

pH

6.6-7.0 (25 °C, 26.5 g/L in H₂O)

solubility

26.5 g/L

application(s)

food and beverages
microbiology

compatibility

for use with E.coli / Coliform Selective-Supplement (1.00898)

storage temp.

15-25°C

suitability

Escherichia coli, coliforms

Popis

General description

Chromocult^® Coliform Agar is a granulated, less selective, and differential chromogenic culture medium intended for microbiological analysis of food and water. This medium saves time by enabling the simultaneous detection, differentiation, and enumeration of E. coli and coliforms from drinking water and processed food matrices within 24 hours. This agar is the only chromogenic culture medium which was used for validation to prepare the new ISO 9308-1 (2014) version. The medium contains Tergitol^™ 7 as an inhibitor of Gram-positive bacteria without any impact on the growth of the targeted coliforms/ E. coli. Chromocult^® Coliform Agar is, therefore, the ideal medium for the detection of coliforms/ E. coli in drinking water and processed foods. It facilitates and speeds up your processes.
E.coli / Coliform Selective-Supplement (1.00898) is available to enhance selectivity and growth conditions.

Application

Chromocult^® Coliform Agar is used for the simultaneous detection of coliform bacteria and E. coli in drinking water, disinfected pool water, finished water from treatment plants and processed food samples.

Analysis Note

Appearance (clearness): slightly turbid to turbid
Appearance (colour): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Enterobacter aerogenes ATCC 13048):
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Enterobacter aerogenes ATCC 13048):
Colony count (Citrobacter freundii ATCC 43864 (WDCM 00006)):
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Enterobacter aerogenes ATCC 13048): ≥ 70 %
Recovery on test medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≥ 70 %
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): partial inhibition
Growth (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): no limit
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): dark-blue to violet
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): dark-blue to violet
Colonies (Enterobacter aerogenes ATCC 13048 (WDCM 00175)): pink to red
Colonies (Citrobacter freundii ATCC 43864 (WDCM 00006)): pink to red
Colonies (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): colourless
Incubation: 21 ± 3 hours at 36 ± 2 °C
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Legal Information

AOAC is a registered trademark of AOAC International

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

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Tato položka	1.00850	1.46758	1.20596
Millipore 1.10426 Chromocult^® Coliform Agar	Millipore 1.00850 Chromocult^® Coliform Agar ES	Millipore 1.46758 Chromocult^® Coliform Agar Kit	Millipore 1.20596 Chromogenic Cronobacter Isolation agar
application(s) food and beverages microbiology	application(s) food and beverages, microbiology	application(s) filtration food and beverages microbiology	application(s) environmental food and beverages microbiology
suitability Escherichia coli, coliforms	suitability Escherichia coli, coliforms	suitability Escherichia coli, coliforms	suitability Cronobacter spp. (spp.)
agency AOAC^®, according to ISO 9308, EPA	agency -	agency ISO 9308	agency GB (4789), according to ISO 22964
manufacturer/tradename ChromoCult^®	manufacturer/tradename ChromoCult^®	manufacturer/tradename ReadyPlate^™	manufacturer/tradename ChromoCult^®
form granular	form granular	form plate (ready-to-use)	form granular
technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) -	technique(s) microbiological culture: suitable

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Skladovací třída

11 - Combustible Solids

wgk

WGK 2

Osvědčení o analýze (COA)

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Globální číslo obchodní položky

Skladová položka	GTIN
1104260500	04022536124612

Questions

1–2 of 2 Questions

2 years ago

Wie wird der Agar aus dem Pulver hergestellt. Muss autoklaviert werden?

1 answer
1. Technical Support
  
  2 years ago
  
  Preparation instructions are as follow:
  Dissolve 26.5 g in 1 liter of purified water. Heat in boiling water and agitate frequently until completely dissolved. Do not autoclave, do not overheat!
  Cool the medium to 45 - 50 °C and pour plates. According to EN ISO 9308-1 dispense in Petri dishes to a depth of at least 4 mm (approximately 18 ml in a 90 mm plate).
  For using by poured plate method cool the medium to 44 - 47 °C in a water bath before use (see EN ISO 11133).
  The prepared medium is slightly turbid to turbid and yellowish. The pH value at 25 °C is in the range of 6.6-7.0
  There should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture, following the procedure as described by EN ISO 11133.
  Preparation and addition of optional Cefsulodin solution (US-EPA approved method): Dissolve 5 mg of Cefsulodin in 2 ml of purified water and sterilize by membrane filtration (0.2 μm nominal pore size). Aseptically add the solution (2 ml per 500 ml of medium) to 500 ml of liquefied medium (45 - 50 °C). Pour plates as described above.
  
  Helpful?
3 years ago

What precautions do you need to take to produce chromocult Coliformes agar, since it cannot be overheated or autoclaved? What are the recommendations? Sterile water? Sterile glassware?

1 answer
1. Technical Support
  
  3 years ago
  
  Ideally, a boiling hot water bath would be best. However, you may also use a hot plate with a magnetic stirrer to prepare the medium. Using a magnetic stir bar, thoroughly mix the powdered media with purified water until no clumps are visible. Use a container larger than the volume being prepared (for example - 1L Erlinmeyer flask to prepare 500 mL media). Heat media to 100°C, which will be the boiling temperature. Boiling will melt the agar, but bubbling will occur. Closely monitor to prevent boiling over. Media is sufficiently heated when the appearance transitions from cloudy/hazy to transparent. Allow media to cool to 45 - 50°C and pour aseptically onto plates. For larger volumes, the media can be dispensed by using a peristaltic pump, Sterile plates, utensils, and/or tubing may be used during the preparation and filling process.
  
  Helpful?

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