The antibody exhibits species cross-reactivity with rat and mouse. It is also predicted to react with human based on 100% sequence homology. Further information may be found in the following citation: Cheong RY, Czieselsky K, Porteous R, Herbison AE. Expression of ESR1 in Glutamatergic and GABAergic Neurons Is Essential for Normal Puberty Onset, Estrogen Feedback, and Fertility in Female Mice. J Neurosci. 2015 Oct 28;35(43):14533-43. doi: 10.1523/JNEUROSCI.1776-15.2015. PMID: 26511244; PMCID: PMC6605465.
06-935
Anti-Estrogen Receptor α Antibody
from rabbit, purified by affinity chromatography
Synonyma:
ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
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Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 100 μg | Očekávané datum odeslání27. dubna 2026odAreál Kühne+Nagel spol. s r.o. | 11 500,00 Kč |
O této položce
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, IP, WB
Citations:
152
11 500,00 Kč
Očekávané datum odeslání27. dubna 2026podrobné informace
Technický servis
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rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), rat (immunogen homology)
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ESR1(2099)
mouse ... Esr1(13982)
rat ... Esr1(24890)
General description
Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
~58 kDa observed. UniProtKB/Swiss-Prot P06211 (ESR1_RAT) lists a molecular weight of 67 kDa A molecular weight between 52-58 kDA due to variant isoforms may be observed in some cell lysates (Al-Bader, M.D. (2006). Reprod Biol Endocrinol. 4:13.; Geisler, J.G. et al. (2002). Diabetes. 51(7):2158-2169.). Uncharacterized bands may be seen at a molecular weight of ~10 kDa, ~35 kDa, ~41 kDa, ~105 kDa, and ~220 kDa in some cell lysates.
Immunogen
Epitope: C-terminus.
KLH-conjugated linear peptide corresponding to the C-terminus of rat Estrogen Receptor alpha.
Application
- Tested applications
- Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Estrogen Receptor in human stomach, human uterine cancer, human breast cancer and human cervical cancer tissue sections.
- Immunoprecipitation Analysis: 4 µg from a representative lot immunoprecipitated Estrogen Receptor in MCF-7 cell lysate.
- Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Estrogen Receptor in MCF-7 cells.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. "
Anti-Estrogen Receptor , Cat. No. 06-935, is a rabbit polyclonal antibody that detects Estrogen Receptor and is tested for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, and Immunohistochemistry (Paraffin).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Hormones & Receptors
Hormones & Receptors
Biochem/physiol Actions
This antibody recognizes the C-terminus of Estrogen Receptor alpha.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
MCF7 cell lysate
MCF7 cell lysate
Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor in MCF-7 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
Tato položka | |||
|---|---|---|---|
| biological source rabbit | biological source mouse | biological source rabbit | biological source rabbit |
| clone polyclonal | clone F3-A, monoclonal | clone 60C, monoclonal | clone 68-4, monoclonal |
| species reactivity human | species reactivity rat, human, mouse | species reactivity rat, pig, mouse, human, bovine | species reactivity mouse, rat, human |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form affinity isolated antibody | antibody form ascites fluid | antibody form culture supernatant | antibody form culture supernatant |
| Gene Information human ... ESR1(2099) | Gene Information human ... ESR1(2099) | Gene Information bovine ... Esr1(407238) | Gene Information human ... ESR2(2100) |
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Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
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Související obsah
A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.
Neurochemical characterization and sexual dimorphism of projections from the brain to abdominal and subcutaneous white adipose tissue in the rat.
Adler, ES; Hollis, JH; Clarke, IJ; Grattan, DR; Oldfield, BJ
The Journal of Neuroscience null
Transcriptional regulation by a naturally occurring truncated rat estrogen receptor (ER), truncated ER product-1 (TERP-1).
Schreihofer, D A, et al.
Molecular Endocrinology, 13, 320-329 (1999)
Specific modulation of estrogen receptor mRNA isoforms in rat pituitary throughout the estrous cycle and in response to steroid hormones.
Friend, K E, et al.
Molecular and cellular endocrinology, 131, 147-155 (1997)
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Will the antibody 06-935, which is listed as having human reactivity in the website information, be suitable for staining in mouse tissue?
1 answer-
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