05-665

Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7

clone 8E7, Upstate^®, from mouse

• Synonyma: Anti-Anti-CTNNB, Anti-Anti-EVR7, Anti-Anti-MRD19, Anti-Anti-NEDSDV, Anti-Anti-armadillo
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 8E7, monoclonal
• Application: FACS, ICC, IHC (p), WB
• Citations: 506

Vlastnosti

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8E7, monoclonal
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001895
• UniProt accession no.: P35222
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Popis

General description

92 kDa

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins.
When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC).
Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Immunogen

Epitope: The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.

Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag

Application

Immunocytochemistry: This antibody was used in immunocytochemistry and showed positive staining on the membrane and cytosol.
This antibody has also been reported by an independent laboratory to show positive immunostaining for beta-catenin in LiCl-treated 293T cells fixed with methanol (Staal, Frank J. T., 2002).

Flow Cytometry: This antibody was used in flow cytometry at an optimal 1 μg/mL concentration.

Immunohistochemistry: This antibody was used in immunohistochemistry on a colorectal carcinoma tissue array at a 1:300 dilution.
This antibody has also been reported by an independent laboratory to detect beta-catenin in mouse embryo sections (Van Noort, M., 2002).

Anti-Active-β-Catenin (anti-ABC) Antibody, clone 8E7 is a well characterized Mouse Monoclonal Antibody. This highly published mAb also known as Anti-Catenin beta-1 readily detects beta-Catenin & has been validated in FC, ICC, IHC, IHC(P) & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Biochem/physiol Actions

Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.

The antibody eptiope is 100% conserved in zebrafish.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1k in buffer containing 0.1 M Tris-Glycine, pH 7.4, 15 mM NaCl and 0.05% sodium azide

Preparation Note

Maintain for 1 year at 2-8°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Routinely evaluated by immunoblot on RIPA lysates from A431 cells.

Western Blot Analysis: 0.2-2 µg/mL of this antibody detected β-catenin in RIPA lysates from A431 cells.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable