CC119
Human Caspase 3, recombinant human active
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About This Item
Código UNSPSC:
12352204
eCl@ss:
32160405
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
human
recombinante
expressed in E. coli
Formulário
solid
fabricante/nome comercial
Chemicon®
técnica(s)
activity assay: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
Informações sobre genes
human ... CASP3(836)
Descrição geral
Specificity: Caspase-3 (also known as CPP32, Yama and apopain) is a member of the interleukin-1beta converting enzyme (ICE) family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (FLICE) granzyme B. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others.
The partially purified* active recombinant human caspase-3 protein with full-length His-6 tag was expressed in E. coli. The expressed Caspase-3 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA). In combination with caspase-3 activity assays, the active recombinant caspase-3 is very useful in the biological screening of caspase inhibitors. The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates.
*10-25% caspase-3 protein by gel analysis, also contains E. Coli proteins. Approximately, 0.8-2 μg total protein / unit of activity. GenBank # U13737.
The partially purified* active recombinant human caspase-3 protein with full-length His-6 tag was expressed in E. coli. The expressed Caspase-3 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA). In combination with caspase-3 activity assays, the active recombinant caspase-3 is very useful in the biological screening of caspase inhibitors. The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates.
*10-25% caspase-3 protein by gel analysis, also contains E. Coli proteins. Approximately, 0.8-2 μg total protein / unit of activity. GenBank # U13737.
Aplicação
Western blot: 1-5 μL of stock per lane. Protein is full length caspase 3 with His-Tag. It is autoactivated thus active caspase subunits will be seen
and 17kDa + 12kDa (large and small subunits) can be seen depending upon the antibody. Small subunit is extremely difficult to detect in most westerns. The size of inactive enzime is ~32 kDa.
Activation: Not needed. When expressed in E. coli, caspase-3 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
and 17kDa + 12kDa (large and small subunits) can be seen depending upon the antibody. Small subunit is extremely difficult to detect in most westerns. The size of inactive enzime is ~32 kDa.
Activation: Not needed. When expressed in E. coli, caspase-3 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
Definição da unidade
Specific Activity: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C at saturated substrate concentrations.
forma física
Lyophilized. Reconstitute to 1 unit/μL with PBS containing 15% glycerol.
Partially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 3 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity.
Partially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 3 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity.
Armazenamento e estabilidade
Maintain unopened and rehydrated product at -70°C in undiluted, freeze-thaw protected, tightly sealed aliquots. Avoid repeated freeze/thaw cycles. Product stable for 6 months from date of purchased if handled appropriately.
Nota de análise
Partially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 3 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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