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P8287

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

chromogenic, tablet

Synonim(y):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

Wzór liniowy:
C6H4(NH2)2·2HCl
Numer CAS:
615-28-1
Masa cząsteczkowa:
181.06
Beilstein:
3912045
Numer MDL:
MFCD00012966
Kod UNSPSC:
12352204
Identyfikator substancji w PubChem:
24898741
NACRES:
NA.83

product name

o-Phenylenediamine dihydrochloride, tablet, 10 mg substrate per tablet

Postać

tablet

mp

258 °C (dec.) (lit.)

temp. przechowywania

2-8°C

ciąg SMILES

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

Klucz InChI

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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Opis ogólny

o-Phenylenediamine (o-PD) is a colorless and nonfluorescent molecule.
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Zastosowanie

o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA).

Rekonstytucja

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
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Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H302 + H332,H317,H319,H341,H351,H410

Klasyfikacja zagrożeń

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Kod klasy składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Klasa zagrożenia wodnego (WGK)

WGK 1

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Sara Buezo Montero et al.
Frontiers in cellular and infection microbiology, 10, 377-377 (2020-08-28)
Mosquitoes of the Aedes genus transmit arboviruses of great importance to human health as dengue, chikungunya, Zika and yellow fever. The tiger mosquito Aedes albopictus can play an important role as arboviral vector, especially when Aedes aegypti is absent or
Henry Surendra et al.
Malaria journal, 18(1), 227-227 (2019-07-10)
Analysis of anti-malarial antibody responses has the potential to improve characterization of the variation in exposure to infection in low transmission settings, where conventional measures, such as entomological estimates and parasitaemia point prevalence become less sensitive and expensive to measure.
Rafaella P M Guimarães-Peixoto et al.
Veterinary parasitology, regional studies and reports, 20, 100375-100375 (2020-05-26)
Bovine cysticercosis remains as one of the most important cause of carcasses and viscera condemnation in Brazilian slaughterhouses. However, the efficiency of post-mortem inspection for the diagnosis of this zoonotic disease is relatively low, and few available studies were performed
Saima Ajaz et al.
American journal of physiology. Cell physiology, 320(1), C57-C65 (2020-11-06)
The COVID-19 pandemic has been the primary global health issue since its outbreak in December 2019. Patients with metabolic syndrome suffer from severe complications and a higher mortality rate due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We
Rama Devudu Puligedda et al.
Antibodies (Basel, Switzerland), 9(1) (2020-03-04)
Poliovirus (PV)-specific intestinal IgAs are important for cessation of PV shedding in the gastrointestinal tract following an acute infection with wild type or vaccine-derived PV strains. We sought to produce IgA monoclonal antibodies (mAbs) with PV neutralizing activity. We first
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