P6662

o-Phenylenediamine dihydrochloride

tablet, 1 mg substrate per tablet

• Synonim(y): 1,2-Phenylenediamine tablet, OPD, OPD Tablet
• Wzór liniowy: C₆H₄(NH₂)₂·2HCl
• Numer CAS: 615-28-1
• Masa cząsteczkowa: 181.06
• UNSPSC Code: 12352204
• NACRES: NA.83
• PubChem Substance ID: 24898787
• MDL number: MFCD00012966
• Beilstein/REAXYS Number: 3912045

Właściwości

• Quality Level: 200
• form: tablet
• mp: 258 °C (dec.) (lit.)
• solubility: water: 1 tablet/10 mL, clear, colorless
• storage temp.: 2-8°C
• SMILES string: Cl[H].Cl[H].Nc1ccccc1N
• InChI: 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• InChI key: RIIWUGSYXOBDMC-UHFFFAOYSA-N

Opis

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08,GHS07,GHS09
• signalword: Warning
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• hcodes: H317,H341,H351,H411
• pcodes: P202 - P261 - P273 - P280 - P302 + P352 - P308 + P313
• Hazard Classifications: Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1