Przejdź do zawartości
Merck
Wszystkie zdjęcia(2)

Dokumenty

P7288

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

chromogenic, tablet

Synonim(y):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(2)

About This Item

Wzór liniowy:
C6H4(NH2)2·2HCl
Numer CAS:
615-28-1
Masa cząsteczkowa:
181.06
Beilstein:
3912045
Numer WE:
210-418-7
Numer MDL:
MFCD00012966
Kod UNSPSC:
12352204
Identyfikator substancji w PubChem:
24898837
NACRES:
NA.83

product name

o-Phenylenediamine dihydrochloride, tablet, 20 mg substrate per tablet

Postać

tablet

mp

258 °C (dec.) (lit.)

temp. przechowywania

2-8°C

ciąg SMILES

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

Klucz InChI

RIIWUGSYXOBDMC-UHFFFAOYSA-N

Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów

Powiązane kategorie

Opis ogólny

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Zastosowanie

o-Phenylenediamine dihydrochloride has been used:
  • as a substrate for the eosinophil peroxidase (EPO) assay in bronchoalveolar lavage fluid (BALF)
  • in cell adhesion molecule-enzyme immunoassay (CAM-EIA) to analyze the amounts of E-selectin induced by test compounds (LPS or solutions and materials) on endothelial cells
  • in ultra-sensitive enzyme-linked immunosorbent assay (ELISA) to measure prolactin and luteinizing hormone (LH)

Rekonstytucja

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H302 + H332,H317,H319,H341,H351,H410

Klasyfikacja zagrożeń

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Hollian R Phillipps et al.
Journal of neuroendocrinology, 34(6), e13129-e13129 (2022-05-02)
Throughout the reproductive cycle in rodents, prolactin levels are generally low. In some species, including rats, a prolactin surge occurs on proestrus with peak concentrations coinciding with the preovulatory luteinizing hormone (LH) surge. In mice, however, there are conflicting reports
D Sano et al.
Journal of applied microbiology, 109(6), 1923-1928 (2010-08-26)
Transmission routes of noroviruses, leading aetiological agents of acute gastroenteritis, are rarely verified when outbreaks occur. Because the destination of norovirus particles being firmly captured by micro-organisms could be totally different from that of those particles moving freely, micro-organisms with
Ronald E Unger et al.
Biomaterials, 35(10), 3180-3187 (2014-01-25)
Endotoxin, also known as lipopolysaccharide (LPS) produced by bacteria can be present in any liquid or on any biomaterial even if the material is sterile. Endotoxin in mammals can cause fever, inflammation, cell and tissue damage and irreversible septic shock
R W Pero et al.
Molecular and cellular biochemistry, 193(1-2), 119-125 (1999-05-20)
Our laboratory has concentrated on the possible regulation the benzamides and nicotinamides may have on the processes of DNA repair and apoptosis. Recent reports have suggested that both apoptosis and inflammation are regulated by the transcription factor NF-kappaB. We have
C A Ison
Methods in molecular medicine, 66, 317-329 (2001-01-01)
Neisseria meningitidis is an obligate human pathogen. When it interacts with the host, it can establish a commensal relationship or can, on a minority of occasions, invade and cause systemic disease. Protection against systemic disease, particularly for serogroup A and
Wyświetl wszystkie powiązane dokumenty

Produkty

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej