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P4069

Phosphatase, Alkaline from Escherichia coli

Name: Phosphatase, Alkaline from <I>Escherichia coli</I>
Brand: SIGMA
Price: 1520 PLN

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Synonim(y):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Gabaryty przesyłki	SKU	Dostępność	Cena netto
25 units		Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności	1520,00 zł
100 units		Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności	4440,00 zł

Informacje o tej pozycji

Numer CAS:

9001-78-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-631-4

MDL number:

MFCD00131849

Numer WE:

3.1.3.1 (BRENDA, IUBMB)

eCl@ss:

42010105

Specific activity:

20-50 units/mg protein (in glycine buffer)

Biological source:

Escherichia coli

1520,00 zł

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Właściwości

biological source

Escherichia coli

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

20-50 units/mg protein (in glycine buffer)

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Opis

Application

Phosphatase, Alkaline from Escherichia coli has been used:

in fluorometric assay to determine its enzyme activity
in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl₂, 0.5 mM ZnCl₂, 50% (w/v) glycerol

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.

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Ta pozycja	P4252	P7923	P6774
Sigma-Aldrich P4069 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P4252 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P7923 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P6774 Phosphatase, Alkaline from bovine intestinal mucosa
specific activity 20-50 units/mg protein (in glycine buffer)	specific activity 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)	specific activity ≥4,000 DEA units/mg protein	specific activity ≥2,000 DEA units/mg protein
biological source Escherichia coli	biological source Escherichia coli	biological source -	biological source bovine intestinal mucosa
form buffered aqueous glycerol solution	form ammonium sulfate suspension	form buffered aqueous glycerol solution	form buffered aqueous solution
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
foreign activity DNase, RNase, none detected	foreign activity -	foreign activity -	foreign activity -