P4252

Phosphatase, Alkaline from Escherichia coli

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

• Synonim(y): Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Numer CAS: 9001-78-9
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• EC Number: 232-631-4
• NACRES: NA.54
• MDL number: MFCD00131849
• Numer WE: 3.1.3.1 (BRENDA, IUBMB)
• Specific activity: 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
• Biological source: Escherichia coli

Właściwości

• biological source: Escherichia coli
• Quality Segment: 300
• form: ammonium sulfate suspension
• specific activity: 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
• storage temp.: 2-8°C

Opis

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.

Biochem/physiol Actions

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium[1][2]. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Suspension in 2.5 M (NH₄)₂SO₄

Analysis Note

The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)