A6132
Apyrase from potatoes
ATPase ≥3.0 units/mg protein, lyophilized powder
Synonim(y):
Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-569-8
MDL number:
Biological source:
potato
Przejdź do
Pomoc techniczna
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Pozwól nam pomócbiological source
potato
Quality Level
type
Grade I
form
lyophilized powder
ATPase activity
≥3.0 units/mg protein
composition
Protein, 30-60%
storage temp.
−20°C
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Ta pozycja | |||
|---|---|---|---|
| biological source potato | biological source potato | biological source - | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 300 | Quality Level 400 | Quality Level 300 |
| type Grade I | type - | type Grade VII | type Grade VII |
| ATPase activity ≥3.0 units/mg protein | ATPase activity ≥600 units/mg protein | ATPase activity ≥200 units/mg protein | ATPase activity ≥200 units/mg protein |
Application
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1].
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.
Reaction: ATP → ADP+Pi → AMP+2Pi.
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Packaging
Sold on the basis of ATPase units.
Physical form
Partially purified, lyophilized powder containing potassium succinate
Other Notes
Mixture of high and low ratio isoenzymes.
One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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I Shirakawa et al.
The Journal of experimental biology, 198(Pt 8), 1809-1815 (1995-08-01)
Kinesin is a motor protein that converts chemical energy derived from ATP hydrolysis into mechanical work to transport cellular components along microtubules. We studied the properties of ATP-dependent microtubule-kinesin sliding with two different in vitro assay systems. In one assay
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Food deprivation can rescue obesity and overweight-induced mood disorders, and promote mood performance in normal subjects. Animal studies and clinical research have revealed the antidepressant-like effect of calorie restriction, but little is known about the mechanism of calorie restriction-induced mood
Robert A Amos et al.
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Rhamnogalacturonan I (RG-I) is a major plant cell wall pectic polysaccharide defined by its repeating disaccharide backbone structure of [4)-α-D-GalA-(1,2)-α-L-Rha-(1,]. A family of RG-I:Rhamnosyltransferases (RRT) has previously been identified, but synthesis of the RG-I backbone has not been demonstrated in
J Vilmart-Seuwen et al.
The Journal of cell biology, 103(4), 1279-1288 (1986-10-01)
We have tried to specify a widespread hypothesis on the requirement of ATP for exocytosis (membrane fusion). With Paramecium tetraurelia cells, synchronously (approximately 1 s) exocytosing trichocysts, ATP pools have been measured in different strains, including wild type cells, "non-discharge"
Avanish Rai et al.
Bio-protocol, 11(1), e3874-e3874 (2021-03-19)
Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP;
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